Lactobacillus farciminis treatment attenuates stress-induced overexpression of Fos protein in spinal and supraspinal sites after colorectal distension in rats
Lactobacillus farciminis treatment attenuates stress-induced overexpression of Fos protein in spinal and supraspinal sites after colorectal distension in rats
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DOI：
10.1111/j.1365-2982.2009.01280.x
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发表时间：
2009-05-01
影响因子：
3.5
通讯作者：
Theodorou, V.
中科院分区：
文献类型：
--
作者：
Ait-Belgnaoui, A.;Eutamene, H.;Theodorou, V.
肠易激综合征（IBS）常伴有心理困扰，其特点是对肠壁膨胀过敏。一些益生菌能够减轻肠易激综合征的症状，减少动物对机械刺激的内脏运动反应。此外，我们之前的研究表明，乳杆菌farciminis治疗可以消除急性应激引起的结肠直肠膨胀（CRD）的痛感过敏。本研究的目的是确定(i)应激诱导的内脏痛觉过敏是否会改变Fos的表达，Fos是CRD诱导的一般神经元激活的标志，（ii）这种激活可以通过乳杆菌治疗来调节。雌性大鼠经法氏乳杆菌处理后，在部分抑制应激或假抑制应激后进行CRD。免疫组化法检测Fos蛋白表达。CRD或PRS后，Fos在脊髓切片（S1）、孤束核（NTS）、下丘脑室旁核（PVN）和杏仁核内侧核（MeA）表达增加。联合刺激（PRS和CRD）显著增加了骶脊髓段、PVN和MeA中Fos的过表达，但在NTS中没有。相比之下，用L. farciminis预处理可显著降低这些区域Fos阳性细胞的数量。本研究表明，PRS可增强CRD诱导的Fos蛋白在脊髓和棘上水平的表达。farciminis处理抑制了这种增强作用，表明这种益生菌菌株的抗伤害作用是由于减少了应激诱导的脊髓和棘上水平感觉神经元的激活/敏化。
Irritable bowel syndrome (IBS), frequently associated with psychological distress, is characterized by hypersensitivity to gut wall distension. Some probiotics are able to alleviate IBS symptoms and reduce visceromotor response to mechanical stimuli in animals. Moreover, we have previously shown that Lactobacillus farciminis treatment abolished the hyperalgesia to colorectal distension (CRD) induced by acute stress. The aims of the present study were to determine whether (i) stress-induced visceral hyperalgesia modifies the expression of Fos, a marker of general neuronal activation, induced by CRD, (ii) this activation can be modulated by L. farciminis treatment. Female rats were treated by L. farciminis and CRD was performed after partial restraint stress (PRS) or sham-PRS. The expression of Fos protein was measured by immunohistochemistry. After CRD or PRS, Fos expression was increased in spinal cord section (S1), nucleus tractus solitarius (NTS), paraventricular nucleus (PVN) of the hypothalamus, and in the medial nucleus of the amygdala (MeA). The combination of both stimuli, PRS and CRD, markedly increased this Fos overexpression in the sacral spinal cord section, PVN and MeA, but not in NTS. By contrast, a pretreatment with L. farciminis significantly reduced the number of Fos positive cells in these area. This study shows that PRS enhances Fos protein expression induced by CRD at the spinal and supraspinal levels in rats. Lactobacillus farciminis treatment inhibited this enhancing effect, suggesting that the antinociceptive effect of this probiotic strain results from a decrease of the stress-induced activation/sensitization of sensory neurons at the spinal and supraspinal level.