THE USE OF RATS ASSOCIATED WITH A HUMAN FECAL FLORA AS A MODEL FOR STUDYING THE EFFECTS OF DIET ON THE HUMAN GUT MICROFLORA
THE USE OF RATS ASSOCIATED WITH A HUMAN FECAL FLORA AS A MODEL FOR STUDYING THE EFFECTS OF DIET ON THE HUMAN GUT MICROFLORA
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DOI：
10.1111/j.1365-2672.1987.tb02415.x
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发表时间：
1987-07-01
期刊：
影响因子：
--
通讯作者：
COLE, CB
中科院分区：
文献类型：
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作者：
MALLETT, AK;BEARNE, CA;COLE, CB
四种细菌生物转化酶（β-葡糖苷酶，β-葡萄糖醛酸酶、硝酸还原酶和硝基还原酶）在常规植物群大鼠或被混合的人粪便植物群污染的无菌大鼠的盲肠内容物中进行测定，并与新鲜人粪便制备物中存在的活性进行比较。常规植物群大鼠和接种人植物群的大鼠均表现出与人粪便基本相似的酶谱，尽管常规大鼠植物群表现出可忽略的硝酸还原酶活性。酶谱基本上保持不变，在两个人的植物群制剂补充果胶的饮食后，而传统的大鼠植物群响应这种植物细胞壁碳水化合物与硝酸还原酶活性显着增加。结果表明，人类粪便微生物菌群的酶活性可以在与人类肠道细菌的混合种群相关的大鼠中模拟。
The activities of four bacterial biotransformation enzymes (.beta.-glucosidase, .beta.-glucuronidase, nitrate reductase and nitroreductase) were measured in the caecal contents of conventional flora rats or germ-free rats contaminated with a mixed, human faecal flora and compared with activities present in a fresh human stool preparation. Both the conventional flora rats and the rats inoculated with a human flora exhibited an enzyme profile generally similar to that of human faeces, although the conventional rat flora exhibited negligible nitrate reductase activity. The enzyme profile remained essentially unaltered in both human flora preparations following supplementation of the diet with pectin, whereas the conventional rat flora responded to this plant cell wall carbohydrate with a significant increase in nitrate reductase activity. The results demonstrate that enzymic activities of the human faecal microflora can be simulated in rats associated with a mixed population of human intestinal bacteria.