Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase.
Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase.
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DOI：
10.1038/nprot.2009.121
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发表时间：
2009
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞色素P450（P450）酶在类固醇、维生素、致癌物、药物和其他化合物的代谢中是重要的。该领域中常用的两种测定法是生物制剂中总P450和NADPH-P450还原酶的测量。一个详细的协议，提出了测量P450的光谱特性，沿着的协议，用于测量NADPH-P450还原酶的NADPH-细胞色素c还原活性。每个试验可在5-10分钟内完成。提供了方案中特定序列的原理的详细解释，沿着潜在的混淆问题。
Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH–P450 reductase in biological preparations. A detailed protocol is presented for the measurement of P450 by its spectral properties, along with a protocol for measuring NADPH–P450 reductase by its NADPH–cytochrome c reduction activity. Each assay can be completed in 5–10 min. Detailed explanations for the rationale of particular sequences in the protocols are provided, along with potential confounding problems.