Strongyloides stercoralis: Role of antibody and complement in immunity to the third stage larvae in BALB/cByJ mice
Strongyloides stercoralis: Role of antibody and complement in immunity to the third stage larvae in BALB/cByJ mice
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DOI：
10.1006/expr.1996.0035
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发表时间：
1996-04-01
影响因子：
2.1
通讯作者：
Abraham, D
中科院分区：
文献类型：
--
作者：
Brigandi, RA;Rotman, HL;Abraham, D
经粪类圆线虫 L3 免疫的小鼠被证明可以杀死扩散室内 90% 以上的攻击幼虫。本研究的目的是确定负责免疫的宿主成分。将来自未受保护的对照小鼠和受保护的免疫小鼠的血清以25-500μl的剂量在与幼虫攻击相同的时间和地点转移至初始小鼠中。转移少至50μl的免疫血清就能够赋予保护性免疫力。通过将细胞从幼虫微环境中排除或通过在受体小鼠中进行单克隆抗体处理来消耗粒细胞来消除血清转移的免疫力。使用 Protein G 和同种型特异性亲和柱分离特异性抗体同种型。由此产生的转移实验确定 IgM 是负责对粪圆线虫 L3 进行保护性免疫的同种型。进行了体内抗体结合研究，发现在免疫动物中只有 IgM 与感染性 L3 和宿主衍生的 L3 (L3(+)) 表面结合。还发现免疫小鼠的 L3/L3(+) 表面结合的 C3 水平升高。对免疫小鼠进行眼镜蛇毒因子处理以消耗补体，完全消除了 C3 与 L3/L3(+) 表面的结合，并消除了免疫力。因此，IgM、补体和粒细胞对于免疫消除粪圆线虫 L3/L3(+) 是必需的。鉴定 IgM 识别的抗原可能有助于选择可能的候选疫苗。 (C) 1996 学术出版社
Mice immunized against Strongyloides stercoralis L3 were shown to kill greater than 90% of challenge larvae contained within diffusion chambers. The objective of the present study was to identify the host components responsible for immunity. Serum from unprotected, control mice and protected, immune mice in doses of 25-500 mu l was transferred into naive mice at the same time and location as larval challenge. Transfer of as little as 50 mu l of immune serum was able to confer protective immunity, The serum-transferred immunity was ablated by excluding cells from the larval microenvironment or by depleting granulocytes through monoclonal antibody treatment in the recipient mice. Specific antibody isotypes were isolated using protein G and isotype-specific affinity columns. The resulting transfer experiments identified IgM as the isotype responsible for protective immunity to S. stercoralis L3. Antibody binding studies in vivo were performed and only IgM bound to the surface of infective L3 and host-derived L3 (L3(+)) in immune animals. Elevated levels of C3 were also found bound to the surface of L3/L3(+) in immune mice. Cobra venom factor treatment of immunized mice to deplete complement completely eliminated C3 binding to the surface of L3/L3(+) and ablated immunity. Therefore, IgM, complement, and granulocytes are necessary for immune elimination of S. stercoralis L3/L3(+). Identification of antigens recognized by IgM may help select possible vaccine candidates. (C) 1996 Academic Press, Inc.