Characterization of Magnetic Markers for Liquid-Phase Immunoassays Using Brownian Relaxation
Characterization of Magnetic Markers for Liquid-Phase Immunoassays Using Brownian Relaxation
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DOI：
10.1143/jjap.51.023002
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发表时间：
2012-02-01
影响因子：
1.5
通讯作者：
Kandori, Akihiko
中科院分区：
文献类型：
--
作者：
Enpuku, Keiji;Watanabe, Hideki;Kandori, Akihiko
我们的特点是用于生物免疫测定的磁性标记物的基础上布朗弛豫。因为标记物由聚集的纳米颗粒组成，即，磁性纳米团簇，我们首先澄清了它们的磁性使用交流磁化率测量，磁化（M-H曲线），和磁弛豫特性。通过对实验结果的分析，我们得到了免疫分析的关键参数，标记物的流体动力学直径d（h）、磁矩m（B）和各向异性能E-B。由于这些参数在实际样品中是分布的，因此我们在分析中考虑了它们的分布。接下来，我们展示了从不同样品获得的这些参数之间的关系。结果表明，m（B）与d（h）近似成比例地增加。另一方面，未获得m（B）和E-B之间的明确相关性。这些结果与单域纳米颗粒的预期结果非常不同，当磁性标记物用于基于布朗弛豫的免疫测定时必须考虑到这些结果。(C)2012日本应用物理学会
We characterized the magnetic markers used in biological immunoassays based on Brownian relaxation. Because the markers are composed of aggregated nanoparticles, i.e., magnetic nanoclusters, we first clarified their magnetic properties using AC susceptibility measurements, magnetization (M-H) curves, and magnetic relaxation properties. Analyzing the experimental results, we obtained the key parameters for the immunoassay, i.e., hydrodynamic diameter d(h), magnetic moment m(B), and anisotropy energy E-B of the markers. Because these parameters were distributed in practical samples, we took their distribution into account in the analysis. Next, we showed the relationship between these parameters obtained from different samples. It was shown that m(B) increased approximately in proportion to d(h). On the other hand, no clear correlation between m(B) and E-B was obtained. These results were very different from those expected from single-domain nanoparticles and must be taken into account when magnetic markers are used in immunoassays based on Brownian relaxation. (C) 2012 The Japan Society of Applied Physics