Changes in intercellular electrical coupling of smooth muscle accompanying atrophy and hypertrophy.
Changes in intercellular electrical coupling of smooth muscle accompanying atrophy and hypertrophy.
复制标题
平滑肌细胞间电耦合的变化伴随着萎缩和肥大。
DOI：
10.1152/ajpcell.1986.250.2.c292
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Sillin,LF
中科院分区：
文献类型：
--
作者：
Bortoff,A;Sillin,LF
测定了猫圆形肠肌的纵向组织阻抗，该肌肉由于容量超载而肥大，或者由于功能丧失而萎缩。这些条件是通过绕过六只猫的 50 厘米空肠回肠，并在 2-6 个月后，从肥大的功能性肠道的近端空肠和绕过的环的萎缩的近端去除部分而产生的。将阻抗与 15 只正常猫的空肠环肌阻抗进行比较。比组织阻抗通过Tomita方法的修改(J.Physiol.Lond.201:145-159,1969)测定，该方法使用Krebs和Krebs-蔗糖溶液；根据经验发现，在克雷布斯蔗糖溶液中会出现 5% 的组织收缩，对此进行了校正。阻抗值是在 30 Hz 至 30 kHz 之间的 20 个频率处确定的。取30 kHz时的值代表各组织的比肌浆电阻(Rmyo)，而30 Hz与30 kHz的值之间的差值代表比连接电阻(Rj)。 Rmyo 的值（以 omega X cm 为单位）为对照 134 +/- 2，功能性 128 +/- 5，旁路 151 +/- 6（均值平均值 +/- SE）。 Rj 的相应值是对照 173 +/- 15，功能性 96 +/- 27，旁路 340 +/- 75。结电容的计算值（以微法/厘米为单位）为对照 2.66，功能性 6.10，旁路 1.97。通过用 100% CO2 饱和沐浴溶液进行酸解偶联，揭示了 Rj 的 pH 敏感电阻成分，推测可归因于间隙连接。（摘要截断为 250 字）
Longitudinal tissue impedance was determined for cat circular intestinal muscle that was either hypertrophied due to volume overloading or atrophied due to defunctionalization. These conditions were produced by bypassing 50 cm of jejuno-ileum in six cats and, 2-6 mo later, removing segments from the proximal jejunum of the hypertrophied functional gut and from the atrophied proximal end of the bypassed loop. Impedances were compared with those of jejunal circular muscle from 15 normal cats. Specific tissue impedance was determined by a modification of the method of Tomita (J. Physiol. Lond. 201: 145-159, 1969), which employs Krebs and Krebs-sucrose solutions; a tissue shrinkage of 5%, empirically found to occur in Krebs-sucrose solution, was corrected for. Impedance values were determined at 20 frequencies between 30 Hz and 30 kHz. The value at 30 kHz was taken to represent the specific myoplasmic resistance (Rmyo) of each tissue, while the difference between the value of 30 Hz and 30 kHz was taken to represent the specific junctional resistance (Rj). Values (in omega X cm) for Rmyo were control 134 +/- 2, functional 128 +/- 5, bypassed 151 +/- 6 (mean of means +/- SE). Corresponding values for Rj were control 173 +/- 15, functional 96 +/- 27, bypassed 340 +/- 75. Calculated values (in microF/cm) for junctional capacitance were control 2.66, functional 6.10, bypassed 1.97. Acid uncoupling by saturating the bathing solutions with 100% CO2 revealed a pH-sensitive resistive component of Rj, assumed to be attributable to gap junctions.(ABSTRACT TRUNCATED AT 250 WORDS)