Neutrophil chemotaxis and receptor expression in clinical septic shock
Neutrophil chemotaxis and receptor expression in clinical septic shock
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DOI：
10.1007/s00134-004-2175-y
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发表时间：
2004-04-01
影响因子：
38.9
通讯作者：
Baudouin, SV
中科院分区：
文献类型：
--
作者：
Chishti, AD;Shenton, BK;Baudouin, SV
目标。目的：验证感染性休克时中性粒细胞对白介素8(IL-8)趋化作用降低的假说。同时检测中性粒细胞CXC趋化因子受体和黏附分子CD11b的表面表达，并研究其与疾病严重程度、气体交换和受体表达的关系。设计。前瞻性队列临床研究。设置。三级转诊教学医院的重症监护室。病人。感染性休克患者(n=15)和健康对照组(n=8)。测量和结果。每天(连续5天)用流式细胞仪检测趋化因子和β整合素的表面表达。“体外”中性粒细胞对IL-8的趋化作用也在败血症患者和健康对照组之间进行了比较。在整个研究过程中，脓毒症组与健康对照组相比，CXCR2表达显著下降，CD11b表达增加，CXCR1表达不变。CD11b与APACHE II评分呈正相关(p
Objective. To examine the hypothesis that neutrophil chemotaxis to interleukin-8 (IL-8) is reduced in septic shock. Surface expression of neutrophil CXC chemokine receptors and the adhesion molecule CD11b were also examined and associations between disease severity, gas exchange and receptor expression were studied. Design. Prospective cohort clinical study. Setting. Intensive care unit in a tertiary referral teaching hospital. Patients. Patients with septic shock (n=15) and healthy controls (n=8) were studied. Measurements and results. Daily (for 5 consecutive days) flow cytometric measurements of chemokine and beta integrin surface expression. "In vitro" neutrophil chemotaxis to IL-8 was also compared between patients with sepsis and healthy controls. CXCR2 expression significantly fell, CD11b expression increased and CXCR1 expression was unchanged throughout the study in the septic group compared with healthy controls. CD11b positively correlated with increasing APACHE II scores (p