Mg2+ induces a sharp and reversible transition in U1 and U2 small nuclear ribonucleoprotein configurations
Mg2+ induces a sharp and reversible transition in U1 and U2 small nuclear ribonucleoprotein configurations
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Mg2 诱导 U1 和 U2 小核核糖核蛋白构型发生急剧且可逆的转变
DOI：
10.1128/mcb.4.9.1890-1899.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
Philippe Jeanteur
中科院分区：
文献类型：
--
作者：
Isabelle;Reveillaud;M. Lelay;J. Sri;C. Brunel;Philippe Jeanteur
当U1和U2小核核糖核蛋白（snRNP）通过自身免疫抗体保留其免疫沉淀性的程序纯化时（Hinterberger等人，J.Biol.Chem.258：2604 - 2613，1983）进行微球菌核酸酶的广泛消化，我们发现它们的降解模式明显依赖于镁浓度，表明它们经历了深刻的结构修饰。在低Mg 2+（小于或等于5mM）下，两种颗粒仅表现出先前被鉴定为除了U6 snRNA之外的所有snRNA共有的耐核心结构（Liautard等人，J. Mol. 162：623 - 643，1982）。在高Mg2+（大于或等于7 mM）下，U1和U2 snRNP的行为彼此不同。在U1 snRNP中，大多数U1 snRNA序列是受保护的，除了可能参与剪接的10个5 '末端核苷酸和核苷酸102和108之间的短序列。跨越核苷酸60至79的另一区域仅被弱保护。这种结构修饰被证明是可逆的。在U2 snRNP中，U2 snRNA序列在其5 '部分直到核苷酸92保持暴露，并且位于核心结构外部的3'末端发夹变得受保护。
When U1 and U2 small nuclear ribonucleoproteins (snRNPs) purified by a procedure which preserves their immunoprecipitability by autoimmune antibodies (Hinterberger et al., J. Biol. Chem. 258:2604-2613, 1983), were submitted to extensive digestion with micrococcal nuclease, we found that their degradation pattern was sharply dependent upon magnesium concentration, indicating that they undergo a profound structural modification. At low Mg2+ (less than or equal to 5 mM), both particles only exhibit a core-resistant structure previously identified as being common to all but U6 snRNAs (Liautard et al., J. Mol. Biol. 162: 623-643, 1982). At high Mg2+ (greater than or equal to 7 mM), U1 and U2 snRNPs behave differently from one another. In U1 snRNP, most U1 snRNA sequence is protected, except for the 10 5'-terminal nucleotides presumably involved in splicing and a short sequence between nucleotides 102 and 108. Another region spanning nucleotides 60 to 79 is only weakly protected. This structural modification was demonstrated to be reversible. In U2 snRNP, the U2 snRNA sequence remains exposed in its 5' part up to nucleotide 92, and the 3'-terminal hairpin located outside the core structure becomes protected.
T1 RNase 对 u-1 核糖核蛋白颗粒中的 U-1 RNA 进行位点特异性切割。
DOI：
10.1073/pnas.78.3.1562
发表时间：
1981
影响因子：
11.1
作者：
Epstein,P;Reddy,R;Busch,H
通讯作者：
Busch,H
硬皮病-多发性肌炎重叠综合征患者的 U2 小核核糖核蛋白自身抗体。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mimori,T;Hinterberger,M;Pettersson,I;Steitz,JA
通讯作者：
Steitz,JA