IGF2 modulates the microenvironment for osteoclastogenesis.
IGF2 modulates the microenvironment for osteoclastogenesis.
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DOI：
10.1016/j.bbrc.2008.11.083
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发表时间：
2009-01
影响因子：
3.1
通讯作者：
Kimihisa Nakao;Mineyoshi Aoyama;Hayato Fukuoka;M. Fujita;K. Miyazawa;K. Asai;S. Goto
中科院分区：
文献类型：
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作者：
Kimihisa Nakao;Mineyoshi Aoyama;Hayato Fukuoka;M. Fujita;K. Miyazawa;K. Asai;S. Goto
我们以前报道过低氧应激通过增加胰岛素样生长因子2（IGF 2）的产生来增强破骨细胞的分化。然而，IGF 2刺激的潜在机制仍然未知。在这项研究中，我们研究了破骨细胞生成的分子机制IGF 2治疗。用IGF 2培养原代小鼠骨髓细胞。将总RNA应用于DNA微阵列分析，然后使用定量RT-PCR来确认微阵列数据并阐明哪些细胞表达相关基因。最有趣的发现是IGF 2处理后基质细胞中CXC趋化因子配体7（CXCL 7）表达和成骨细胞中基质细胞衍生因子1（SDF 1）表达上调。CXCL 7中加入外源性SDF 1可增加破骨细胞的数量，促进巨破骨细胞的形成。这些结果表明，IGF 2调节破骨细胞前体细胞周围的微环境。SDF 1和CXCL 7可能共同促进巨破骨细胞的形成。
We previously reported that hypoxic stress enhanced osteoclast differentiation via increasing insulin-like growth factor 2 (IGF2) production. However, the mechanisms underlying IGF2 stimulation remains unknown. In this study, we investigated the molecular mechanisms of osteoclastogenesis by IGF2 treatment. Primary mouse bone marrow cells were cultured with IGF2. Total RNAs were applied to a DNA microarray analysis, and quantitative RT-PCR was then used to confirm the microarray data and clarify which cells expressed the relative genes. The most interesting findings were the upregulations of CXC chemokine ligand 7 (CXCL7) expression in stromal cells and stromal cell-derived factor 1 (SDF1) expression in osteoblastic cells with IGF2 treatment. The addition of exogenous SDF1 to CXCL7 increased the number of osteoclasts and promoted the formation of giant osteoclasts. These results suggest that IGF2 modulates the microenvironment around osteoclast precursor cells. SDF1 together with CXCL7 may promote the formation of giant osteoclasts.