Characterization of a microbial community capable of nitrification at cold temperature
Characterization of a microbial community capable of nitrification at cold temperature
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DOI：
10.1016/j.biortech.2009.07.091
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发表时间：
2010-01-01
影响因子：
11.4
通讯作者：
Ellison, Aprel Q.
中科院分区：
文献类型：
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作者：
Ducey, Thomas F.;Vanotti, Matias B.;Ellison, Aprel Q.
虽然氨氧化是先进需氧牲畜废水处理的一个组成部分，但氨氧化细菌的硝化速率在较低温度下会大大降低。在这项研究中，我们报告了一种适应的泻湖硝化污泥，它能够在 5 摄氏度（11.2 mg N/g MLVSS/h）至 20 摄氏度（40.4 mg N/g MLVSS/h）的温度下进行高硝化速率。通过部分 16S rRNA 基因测序研究了硝化污泥中微生物群落的组成。提取 DNA 并创建质粒文库后，对 153 个部分长度 16S rRNA 基因克隆进行了测序和系统发育分析。这些克隆中超过 80% 属于变形菌门，并分为 β 类（114 个克隆）、γ 类（7 个克隆）和 α 类（2 个克隆）。其余克隆属于酸杆菌门（1 个克隆）、放线菌门（8 个克隆）、拟杆菌门（16 个克隆）和疣微菌门（5 个克隆）。大多数克隆属于亚硝化单胞菌属，而其他克隆则属于先前鉴定为具有絮状形成或耐冷特性的微生物。由爱思唯尔有限公司出版
While the oxidation of ammonia is an integral component of advanced aerobic livestock wastewater treatment, the rate of nitrification by ammonia-oxidizing bacteria is drastically reduced at colder temperatures. In this study we report an acclimated lagoon nitrifying sludge that is capable of high rates of nitrification at temperatures from 5 degrees C (11.2 mg N/g MLVSS/h) to 20 degrees C (40.4 mg N/g MLVSS/h). The composition of the microbial community present in the nitrifying sludge was investigated by partial 16S rRNA gene sequencing. After DNA extraction and the creation of a plasmid library, 153 partial length 16S rRNA gene clones were sequenced and analyzed phylogenetically. Over 80% of these clones were affiliated with the Proteobacteria, and grouped with the beta- (114 clones), gamma- (7 clones), and alpha-classes (2 clones). The remaining clones were affiliated with the Acidobacteria (1 clone), Actinobacteria (8 clones), Bacteroidetes (16 clones), and Verrucomicrobia (5 clones). The majority of the clones belonged to the genus Nitrosomonas, while other clones affiliated with microorganisms previously identified as having floc forming or psychrotolerance characteristics. Published by Elsevier Ltd.