Prevalence of antibodies to hepatitis E virus among Japanese blood donors: Identification of three blood donors infected with a genotype 3 hepatitis E virus
Prevalence of antibodies to hepatitis E virus among Japanese blood donors: Identification of three blood donors infected with a genotype 3 hepatitis E virus
复制标题
DOI：
10.1002/jmv.20125
复制
发表时间：
2004-08-01
影响因子：
12.7
通讯作者：
Okamoto, H
中科院分区：
文献类型：
--
作者：
Fukuda, S;Sunaga, J;Okamoto, H
在包括日本在内的工业化国家中，个人感染戊型肝炎的危险因素尚不完全清楚。我们调查了丙氨酸转氨酶 (ALT) 水平升高或不升高的日本献血者是否可能感染戊型肝炎病毒 (HEV)。在两个日本红十字血液中心采集了 5,343 名无偿献血者的血清样本，其中包括 1,087 名 ALT 升高至 61-966 IU/L 的献血者和 4,256 名 ALT 正常（低于或等于 60 IU/L）的献血者，并通过内部酶联免疫吸附测定 (ELISA) 检测是否存在抗 HEV IgG。总体而言，200 名捐献者 (3.7%) 的抗 HEV IgG 呈阳性，其中 32 名 (2.9%) 的 ALT 升高，168 名 (3.9%) 的 ALT 正常。通过内部 ELISA 进一步测试含有抗 HEV IgG 的血清样本中是否存在抗 HEV IgM，并通过逆转录 (RT)-聚合酶链式反应 (PCR) 测试 HEV RNA 的存在。三名 ALT 为 966、62 或 61 IU/L 的捐献者抗 HEV IgM 和 HEV RNA 呈阳性。从分离为基因型3的三个病毒血症供体获得的HEV分离株在开放阅读框2的412个核苷酸序列中彼此相似性为91.5-93.4%，并且与从从未出国的日本散发性急性戊型肝炎患者中回收的JRA1分离株具有最高的一致性，为91.5-94.9%，表明这三个HEV分离株是日本本土的。这项研究表明，日本有或没有升高 ALT 的献血者中有一小部分但比例很大，患有病毒血症，并可能导致输血相关的戊型肝炎。 (C) 2004 Wiley-Liss, Inc.
Risk factors for acquiring hepatitis E among individuals in industrialized countries including Japan are not fully understood. We investigated whether Japanese blood donors with or without an elevated alanine aminotransferase (ALT) level are likely to have hepatitis E virus (HEV) infection. Serum samples were collected from 5,343 voluntary blood donors including 1,087 donors with elevated ALT of 61-966 IU/L and 4,256 donors with normal ALT ( less than or equal to 60 IU/L) at two Japanese Red Cross Blood Centers, and were tested for the presence of anti-HEV IgG by in-house enzyme-linked immunosorbent assay (ELISA). Overall, 200 donors (3.7%) were positive for anti-HEV IgG, including 32 (2.9%) with elevated ALT and 168 (3.9%) with normal ALT. Serum samples with anti-HEV IgG were further tested for the presence of anti-HEV IgM by in-house ELISA and for HEV RNA by reverse transcription (RT)-polymerase chain reaction (PCR). Three donors with ALT of 966, 62 or 61 IU/L were positive for anti-HEV IgM and HEV RNA. The HEV isolates obtained from the three viremic donors segregated into genotype 3, were 91.5-93.4% similar to each other in the 412 nucleotide sequence of open reading frame 2, and had the highest identity of 91.5-94.9% with the JRA1 isolate which was recovered from a Japanese patient with sporadic acute hepatitis E who had never been abroad, suggesting that these three HEV isolates are indigenous to Japan. This study suggests that a small but significant proportion of blood donors in Japan with or without elevated ALT are viremic and are potentially able to cause transfusion-associated hepatitis E. (C) 2004 Wiley-Liss, Inc.