Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing
Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing
复制标题
壳聚糖/胶原组装的仿生LBL结构纳米纤维基质促进伤口愈合
DOI：
10.1016/j.biomaterials.2015.02.076
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发表时间：
2015-06-01
期刊：
影响因子：
14
通讯作者：
Li, Xueyong
中科院分区：
文献类型：
--
作者：
Huang, Rong;Li, Wangzhou;Li, Xueyong
本文报道了通过聚己内酯（PCL）/醋酸纤维素（CA）共静电纺丝和带正电荷的壳聚糖（CS）和带负电荷的I型胶原在纳米纤维基质上的层层自组装（LBL）制备仿生纳米纤维基质。FE-SEM图像表明，当涂层双层从5到20.5变化时，平均纤维直径从392增加到541 nm。此外，MIT和SEM结果证实了所制备的纳米纤维垫具有良好的生物相容性和增强的正常人皮肤成纤维细胞（NHDF）的附着和铺展。此外，LBL结构化的（CS/胶原）（n）纳米纤维垫极大地改善了体外细胞迁移，促进了体内再上皮化和血管化，并上调了胶原IV和α-微管蛋白的表达，以及整合素β 1和粘着斑激酶（FAK）在Tyr-397处的磷酸化。通过免疫组织化学和蛋白质印迹分析，表达的蛋白质水平随着包被双层的增加而显著增强。总的来说，这些结果表明，LBL结构的仿生纳米纤维基质可以通过上调ECM蛋白的分泌和触发整合素/FAK信号通路来增强细胞迁移并进一步促进皮肤再生，这证明了纳米纤维垫快速恢复受伤皮肤的结构和功能特性的潜在用途。(C)2015爱思唯尔有限公司版权所有。
This paper reports the fabrication of biomimetic nanofibrous matrices via co-electrospinning of polycaprolactone (PCL)/cellulose acetate (CA) and layer-by-layer self-assembly (LBL) of positively charged chitosan (CS) and negatively charged Type I collagen on the nanofibrous matrix. FE-SEM images indicate that the average fiber diameter increased from 392 to 541 nm when the coating bilayers varied from 5 to 20.5. Besides, the excellent biocompatibility and enhanced attachment and spreading of normal human dermal fibroblasts (NHDFs) of prepared nanofibrous mats are confirmed by MIT and SEM results. Furthermore, the LBL structured (CS/collagen)(n) nanofibrous mats greatly improve the cell migration in vitro, promote re-epithelialization and vascularization in vivo, and up-regulate the expression of collagen IV and alpha-tubulin, as well as the Integrin beta 1 and phosphorylation of focal adhesion kinase (FAK) at Tyr-397. The levels of expressed protein are significantly enhanced with increasing coating bilayers via immunohistochemistry and western blotting analyses. Collectively, these results suggest that the LBL structured biomimetic nanofibrous matrices may enhance cell migration and further promote the skin regeneration by up-regulating the secretion of ECM protein and triggering Integrin/FAK signaling pathway, which demonstrate the potential use of the nanofibrous mats to rapidly restore the structural and functional properties of wounded skin. (C) 2015 Elsevier Ltd. All rights reserved.