Resveratrol, a natural product derived from grapes, is a new inducer of differentiation in human myeloid leukemias
Resveratrol, a natural product derived from grapes, is a new inducer of differentiation in human myeloid leukemias
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DOI：
10.1007/bf02982118
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发表时间：
2002-06-01
影响因子：
2.1
通讯作者：
Koeffler, HP
中科院分区：
文献类型：
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作者：
Asou, H;Koshizuka, K;Koeffler, HP
天然产物白藜芦醇（3,4,40-三羟基反式二苯乙烯）是葡萄和其他食品中发现的一种植物抗菌素，被称为癌症化学预防剂。本实验通过对17例急性髓系白血病患者的髓系细胞（HL-60、NB4、U937、THP-1、ML-1、Kasumi-1）增殖分化的影响，研究了该化合物的体外生物学活性。白藜芦醇（20mum, 4 d）对6个细胞系的生长均有抑制作用。白藜芦醇（10mum）在除Kasumi-1外的所有细胞系中均增强了粘附分子（CD11a、CD11b、CD18、CD54）的表达。此外，白藜芦醇（25 muM， 4天）诱导37%的U937细胞产生超氧化物，通过降低硝基蓝四氮唑（NBT）的能力来测量。白藜芦醇（10 μ m）和全反式维甲酸（ATRA） （50 nM， 4天）联合使用可诱导95%的NB4细胞变为nbt阳性
A natural product, resveratrol (3,4,40-trihydroxy-trans-stilbene), a phytoalexin found in grapes and other food products, is known as a cancer chemopreventive agent. We studied the in vitro biological activity of this compound by examining its effect on proliferation and differentiation in myeloid leukemia cell lines (HL-60, NB4, U937, THP-1, ML-1, Kasumi-1) and fresh samples from 17 patients with acute myeloid leukemia. Resveratrol (20 muM, 4 days) alone inhibited the growth in liquid culture of each of the 6 cell lines. Resveratrol (10 muM) enhanced the expression of adhesion molecules (CD11a, CD11b, CD18, CD54) in each of the cell lines except for Kasumi-1. Moreover, resveratrol (25 muM, 4 days) induced 37% of U937 cells to produce superoxide as measured by the ability to reduce nitroblue tetrazolium (NBT). The combination of resveratrol (10 muM) and all-trans-retinoic acid (ATRA) (50 nM, 4 days) induced 95% of the NB4 cells to become NBT-positive, whereas