Generation of bat-derived influenza viruses and their reassortants
Generation of bat-derived influenza viruses and their reassortants
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DOI：
10.1038/s41598-018-37830-x
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发表时间：
2019-02-04
影响因子：
4.6
通讯作者：
Takada, Ayato
中科院分区：
文献类型：
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作者：
Sato, Masahiro;Maruyama, Junki;Takada, Ayato
在中美洲和南美洲的果蝠中检测到两种新型甲型流感病毒样基因组。然而，这些蝙蝠衍生的流感病毒（BatIV）的生物学特性在很大程度上仍然是未知的，因为从未从受感染的宿主物种中分离出感染性病毒颗粒。在这项研究中，反向遗传学的方法被用来产生感染性BatIV颗粒完全从质粒编码全长序列的8个基因片段。我们将BatIV颗粒接种到各种细胞培养物中，包括蝙蝠衍生的细胞系，并发现BatIV有效地感染特定的蝙蝠衍生的细胞，但不感染其他测试的细胞系。还成功地产生了两种BatIV之间的回复病毒，并证实了它们在易感蝙蝠细胞系中的复制。这些研究结果表明，有限的宿主范围和BatIV在自然界中的重组潜力，为了解BatIV的生态学提供了基础信息。
Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.