Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model.
Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model.
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DOI：
10.1042/bsr20201532
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发表时间：
2020-10-30
影响因子：
4
通讯作者：
Mullin JM
中科院分区：
文献类型：
--
作者：
Callaghan PJ;Ferrick B;Rybakovsky E;Thomas S;Mullin JM
人支气管上皮细胞系16HBE14o-(16HBE)被广泛用作呼吸道上皮病和屏障功能的模型。在分化过程中，跨上皮电阻(TER)增加到约800欧姆×厘米~2，而14C-d-甘露醇流量(JM)同时下降。扩散势研究表明，紧密连接(TJ)对阴离子具有选择性，Pc1/PNA=1.9。佛波酯、蛋白激酶C激活剂、12-O-十四酰佛波醇-13-乙酸酯和促炎细胞因子肿瘤坏死因子-α可诱导跨皮细胞渗漏。微量营养素、锌或槲皮素不能改善基础屏障功能。具有方法学意义的是，在最初的屏障形成后，TER被观察到更具变异性，并自发地显著降低，而JM则没有明显的波动或增加。与分化过程中TER和JM呈强负相关不同，分化的细胞层不表现出TER和JM的关系。与JM相比，TER值的变异性也大得多。用GlyH-101抑制囊性纤维化跨膜电导调节器(CFTR)氯通道后，短路电流(ISC)显著降低，TER略有升高，而JM无明显变化。不仅对ISC，而且对TER都有很强的温度依赖性。综上所述，利用16HBE作为呼吸道屏障功能研究模型的研究需要意识到，鉴于依赖CFTR的跨细胞电导对TER的影响，TER作为屏障功能参数的复杂性。
The human bronchial epithelial cell line, 16HBE14o- (16HBE), is widely used as a model for respiratory epithelial diseases and barrier function. During differentiation, transepithelial electrical resistance (TER) increased to approximately 800 Ohms × cm2, while 14C-d-mannitol flux rates (Jm) simultaneously decreased. Tight junctions (TJs) were shown by diffusion potential studies to be anion-selective with PC1/PNa = 1.9. Transepithelial leakiness could be induced by the phorbol ester, protein kinase C (PKC) activator, 12-O-tetradecanoylphorbol-13-acetate (TPA), and the proinflammatory cytokine, tumor necrosis factor-α (TNF-α). Basal barrier function could not be improved by the micronutrients, zinc, or quercetin. Of methodological significance, TER was observed to be more variable and to spontaneously, significantly decrease after initial barrier formation, whereas Jm did not significantly fluctuate or increase. Unlike the strong inverse relationship between TER and Jm during differentiation, differentiated cell layers manifested no relationship between TER and Jm. There was also much greater variability for TER values compared with Jm. Investigating the dependence of 16HBE TER on transcellular ion conductance, inhibition of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) chloride channel with GlyH-101 produced a large decrease in short-circuit current (Isc) and a slight increase in TER, but no significant change in Jm. A strong temperature dependence was observed not only for Isc, but also for TER. In summary, research utilizing 16HBE as a model in airway barrier function studies needs to be aware of the complexity of TER as a parameter of barrier function given the influence of CFTR-dependent transcellular conductance on TER.
影响因子：
3.7
作者：
Leung C;Shaheen F;Bernatchez P;Hackett TL
通讯作者：
Hackett TL