SNP-STR analysis for non-invasive paternity test for fetus
SNP-STR analysis for non-invasive paternity test for fetus
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SNP-STR分析用于胎儿无创亲子鉴定
DOI：
10.1016/j.fsigss.2017.09.157
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发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Liang, W. B.
中科院分区：
文献类型：
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作者：
Wang, L.;Li, Y.;Liang, W. B.
孕期母体血浆中存在胎儿游离（cff）DNA，这使得对因性侵犯而怀孕的女性进行无创亲子鉴定成为可能。然而，在大量母体DNA的背景下，胎儿游离DNA不易被检测到。为分析两名男性不平衡混合样本中的微量成分而开发的单核苷酸多态性 - 短串联重复序列（SNP - STR）标记，被用于孕期的无创亲子鉴定。基于美国扩展核心基因座的常染色体STR筛选出6个SNP - STR位点，即rs4847015 - D1S1656、rs6736691 - D2S1338、rs25768 - D5S818、rs7786079 - D7S820、rs2246512 - D10S1248、rs11642858 - D16S539，并通过扩增阻滞突变系统（ARMS）方法设计相应的引物。每个位点的引物由两条针对SNP基因型的正向引物和一条反向引物组成，每条正向引物分别与反向引物进行扩增。这6个位点的扩增产物均小于210bp。通过对母体和生父DNA进行SNP - STR PCR分析，筛选出有效标记，用于检测10名怀孕16 - 22周的孕妇血浆样本中的胎儿游离DNA。结果证明了SNP - STR在无创亲子鉴定中的可用性。
The existence of cell-free fetal (cff) DNA in maternal plasma through the process of pregnancy, makes it possible to do noninvasive paternity test for a woman who got pregnant as a result of a sexual assault. However, the cff DNA in a high background of maternal DNA could not be easily detected. The SNP-STR markers, developed for analyzing the minor component in a two-man unbalanced mixture, were used for noninvasive paternity identification during pregnancy. Six SNP-STR loci, rs4847015-D1S1656, rs6736691-D2S1338, rs25768-D5S818, rs7786079-D7S820, rs2246512-D10S1248, rs11642858-D16S539, were screened based on autosomal STRs of Expanded U.S. Core Loci, and the corresponding primers were designed by amplification refractory mutation system (ARMS) method. The primers for every loci consisted of two forward primers targeting the genotype of SNP and one reverse primer, and each forward primer was amplified with the reverse one respectively. The amplicons of the six loci were all less than 210 bp. The cff DNA of plasma samples from ten pregnant women at 16 to 22-week was detected using the informative makers selected from the analysis of maternal and biological paternal DNA by SNP-STR PCR. The results demonstrate the availability of SNP-STR in the noninvasive paternity test.