Distinct Skeletal Muscle Gene Regulation from Active Contraction, Passive Vibration, and Whole Body Heat Stress in Humans.
Distinct Skeletal Muscle Gene Regulation from Active Contraction, Passive Vibration, and Whole Body Heat Stress in Humans.
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DOI：
10.1371/journal.pone.0160594
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Shields RK
中科院分区：
文献类型：
--
作者：
Petrie MA;Kimball AL;McHenry CL;Suneja M;Yen CL;Sharma A;Shields RK
骨骼肌运动调节人类几个重要的代谢基因。我们对环境应力（热）和机械应力（振动）对骨骼肌的影响知之甚少。被动机械应力或全身热应力通常与许多主动运动计划结合使用。我们设计了一种方法来传递振动应力和全身热应力，以比较主动骨骼肌收缩的效果。目的：本研究的目的是检查主动机械应力（肌肉收缩）、被动机械应力（振动）或全身热应力是否调节与肌肉代谢、肥大/萎缩和炎症/修复相关的关键基因特征。方法：11名受试者，6名健康人和5名慢性脊髓损伤（SCI）患者参加了这项研究。六名身体健全的受试者在热应激室中坐了30分钟。5名SCI受试者接受了单次肢体节段振动或重复电诱导肌肉收缩。每次应激完成后3小时，我们进行肌肉活检（股外侧肌或比目鱼肌）以分析mRNA基因表达。结果如下：我们发现，重复的主动肌肉收缩上调了代谢转录因子NR 4A 3（12.45倍）、PGC-1α（5.46倍）和ABRA（5.98倍）;并抑制了MMP 3（0.56倍）。热应激抑制PGC-1α（0.74倍变化; p < 0.05）;而振动诱导FOXK 2（2.36倍变化; p < 0.05）。振动类似地引起MIBR的下调（0.74倍变化; p < 0.05），但程度低于主动肌肉收缩。振动诱导FOXK 2（p < 0.05），而热应激抑制PGC-1α（0.74倍）和ANKRD 1基因（0.51倍; p < 0.05）。结论：这些发现支持了一个独特的基因调控响应热应激，振动和肌肉收缩。了解这些反应可能有助于开发再生康复干预措施，以改善肌肉细胞的发育，生长和修复。
Skeletal muscle exercise regulates several important metabolic genes in humans. We know little about the effects of environmental stress (heat) and mechanical stress (vibration) on skeletal muscle. Passive mechanical stress or systemic heat stress are often used in combination with many active exercise programs. We designed a method to deliver a vibration stress and systemic heat stress to compare the effects with active skeletal muscle contraction. Purpose: The purpose of this study is to examine whether active mechanical stress (muscle contraction), passive mechanical stress (vibration), or systemic whole body heat stress regulates key gene signatures associated with muscle metabolism, hypertrophy/atrophy, and inflammation/repair. Methods: Eleven subjects, six able-bodied and five with chronic spinal cord injury (SCI) participated in the study. The six able-bodied subjects sat in a heat stress chamber for 30 minutes. Five subjects with SCI received a single dose of limb-segment vibration or a dose of repetitive electrically induced muscle contractions. Three hours after the completion of each stress, we performed a muscle biopsy (vastus lateralis or soleus) to analyze mRNA gene expression. Results: We discovered repetitive active muscle contractions up regulated metabolic transcription factors NR4A3 (12.45 fold), PGC-1α (5.46 fold), and ABRA (5.98 fold); and repressed MSTN (0.56 fold). Heat stress repressed PGC-1α (0.74 fold change; p < 0.05); while vibration induced FOXK2 (2.36 fold change; p < 0.05). Vibration similarly caused a down regulation of MSTN (0.74 fold change; p < 0.05), but to a lesser extent than active muscle contraction. Vibration induced FOXK2 (p < 0.05) while heat stress repressed PGC-1α (0.74 fold) and ANKRD1 genes (0.51 fold; p < 0.05). Conclusion: These findings support a distinct gene regulation in response to heat stress, vibration, and muscle contractions. Understanding these responses may assist in developing regenerative rehabilitation interventions to improve muscle cell development, growth, and repair.