Immunohistochemical study of the 32-kDa galectin (beta-galactoside-binding lectin) in the nematode Caenorhabditis elegans
Immunohistochemical study of the 32-kDa galectin (beta-galactoside-binding lectin) in the nematode Caenorhabditis elegans
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DOI：
10.1007/bf02331444
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Hirano, H
中科院分区：
文献类型：
--
作者：
Arata, Y;Akimoto, Y;Hirano, H
使用抗凝集素抗血清通过免疫组织化学方法检查了线虫秀丽隐杆线虫的 32 kDa 半乳糖苷结合凝集素（β-半乳糖苷结合凝集素）的定位，这是在线虫中发现的第一个凝集素。发现凝集素在成人角质层和咽终球中含量最多。然而，尽管免疫化学实验表明其存在，但很难在幼虫动物中找到半乳糖凝集素。这些结果表明，半乳糖凝集素的基本作用之一可能是作为持久外屏障的组成部分，就像鸡胚胎皮肤的形态发生的情况一样。
The localization of the 32-kDa galectin (beta-galactoside-binding lectin) of the nematode Caenorhabditis elegans, which is the first lectin to be found in a nematode, was examined immunohistochemically using an anti-lectin antiserum. The lectin was found to be localized most abundantly in the adult cuticle and also in the terminal bulb of the pharynx. However, it was difficult to locate the galectin in larval animals, though immunochemical experiments suggested its presence. These results suggest that one of the fundamental roles of the galectin may be as a component of the durable outer barrier, as in the case of the morphogenesis of chick embryonic skin.