FLUOROGENIC REACTION AND SPECIFIC MICRO-DETERMINATION OF AMMONIA
FLUOROGENIC REACTION AND SPECIFIC MICRO-DETERMINATION OF AMMONIA
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DOI：
10.1093/oxfordjournals.jbchem.a133257
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
OYAMA, F
中科院分区：
文献类型：
--
作者：
SUGAWARA, K;OYAMA, F
报道了氨与邻苯二甲醛和二硫苏糖醇的荧光反应。最佳检测波长为λ ex = 413 nm和λ em = 476 nm。这种荧光对氨是特异性的：其他氨基化合物，如氨基酸、胺和蛋白质，在413 nm处激活时，在476 nm处不显示发射。该反应允许生物材料中氨的特定微量测定，低至纳摩尔范围。
The fluorogenic reaction of ammonia with o-phthalaldehyde and dithiothreitol is reported. Optimal detection wavelengths are .lambda.ex = 413 nm and .lambda.em = 476 nm. This fluorescence is specific for ammonia: other amino compounds, such as amino acids, amines and proteins, do not show emission at 476 nm when activated at 413 nm. This reaction permits specific microdetermination of ammonia in biological materials down to the nanomole range.