The affinity of DNA sequences containing R5Y5 motif and TA repeats with 10.5-bp periodicity to histone octamer in vitro
The affinity of DNA sequences containing R5Y5 motif and TA repeats with 10.5-bp periodicity to histone octamer in vitro
复制标题
含有R5Y5基序和10.5 bp周期性TA重复的DNA序列与组蛋白八聚体的体外亲和力
DOI：
10.1080/07391102.2018.1477621
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发表时间：
2019-01-01
影响因子：
4.4
通讯作者：
Cai，Lu
中科院分区：
文献类型：
--
作者：
Zhao，Hongyu;Zhang，Fenghui;Cai，Lu
核小体沿基因组的定位部分取决于组蛋白上固有的DNA序列偏好。trtrifonov等人基于几种理论模型提出的核小体DNA中的RRRRRYYYYY （R5Y5, R =嘌呤和Y =嘧啶）基序，可能是促进核小体组装的序列模式。然而，并没有高一致性的实验证据支持R5Y5基序是决定核小体定位的关键元素的概念。在这项工作中，能力的规范，H2A。采用体外盐透析法研究了含Z-和h3.3的八聚体在含有R5Y5基序和TA重复序列的DNA模板上组装核小体的能力。结果表明，R5Y5基序和TA重复序列沿DNA模板的10.5 bp周期性分布可显著促进典型核小体的组装，可能是典型核小体组装的关键序列因子。与周期在10.5 bp以内的TA重复序列相比，DNA模板中的R5Y5基序没有提升H2A。含Z-和h3.3的核小体体外形成效率。这一结果表明R5Y5基序可能不是调节组蛋白变异核小体组装的关键因素。推测标准组蛋白和变异组蛋白对核小体组装的调节机制不同。这些结论可以为核小体定位的机制提供更深入的了解。由Ramaswamy H. Sarma传达
Abstract Nucleosome positioning along the genome is partially determined by the intrinsic DNA sequence preferences on histone. RRRRRYYYYY (R5Y5, R = Purine and Y = Pyrimidine) motif in nucleosome DNA, which was presented based on several theoretical models by Trifonov et al., might be a facilitating sequence pattern for nucleosome assembly. However, there is not a high conformity experimental evidence to support the concept that R5Y5 motif is a key element for the determination of nucleosome positioning. In this work, the ability of the canonical, H2A.Z- and H3.3-containing octamers to assemble nucleosome on DNA templates containing R5Y5 motif and TA repeats within 10.5-bp periodicity was investigated by using salt-dialysis method in vitro. The results showed that the10.5-bp periodical distributions of both R5Y5 motif and TA repeats along DNA templates can significantly promote canonical nucleosome assembly and may be key sequence factors for canonical nucleosome assembly. Compared with TA repeats within 10.5-bp periodicity, R5Y5 motif in DNA templates did not elevate H2A.Z- and H3.3-containing nucleosome formation efficiency in vitro. This result indicates that R5Y5 motif probably isn’t a pivotal factor to regulate nucleosome assembly on histone variants. It is speculated that the regulatory mechanism of nucleosome assembly is different between canonical and variant histone. These conclusions can provide a deeper insight on the mechanism of nucleosome positioning. Communicated by Ramaswamy H. Sarma