APPLICATION OF THE THIOCARBOHYDRAZIDE-OSMIUM COATING TECHNIQUE TO SCANNING ELECTRON-MICROSCOPY OF THE INNER-EAR
APPLICATION OF THE THIOCARBOHYDRAZIDE-OSMIUM COATING TECHNIQUE TO SCANNING ELECTRON-MICROSCOPY OF THE INNER-EAR
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DOI：
10.3109/00016488109138498
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发表时间：
1981-01-01
影响因子：
1.4
通讯作者：
RESNICK, S
中科院分区：
文献类型：
--
作者：
HEYWOOD, P;RESNICK, S
描述了硫代碳酰肼-Os涂层技术在内耳扫描电子显微镜中的应用。在此过程中，已准备好用于 EM 的标本 [胎儿豚鼠]（在缓冲戊二醛或卡诺夫斯基固定剂中固定，然后在四氧化锇中后固定）两次暴露于硫代碳酰肼和四氧化锇溶液中，每次处理之间用水冲洗。这种方法将 Os 与样品结合，因此无需在材料表面涂覆一层重金属。初步观察表明，这是一种方便可靠的方法，能够产生良好的结果。
The application of the thiocarbohydrazide-Os coating technique to scanning electron microscopy of the inner ear is described. In this procedure specimens [fetal guinea pigs] which have been prepared for EM (fixation in buffered glutaraldehyde or in Karnovsky''s fixative followed by post fixation in Os tetroxide) are twice exposed to solutions of thiocarbohydrazide and Os tetroxide with water rinses in between each treatment. This method binds Os to the specimen and so eliminates the need to coat the surface of the material with a layer of heavy metal. Initial observations indicate that this is a convenient and reliable method which is capable of yielding good results.