The fluorescent capillary fill device
The fluorescent capillary fill device
复制标题
荧光毛细管填充装置
DOI：
10.1016/0925-4005(93)85260-h
复制
发表时间：
1993
影响因子：
8.4
通讯作者：
S. C. Whiteley
中科院分区：
文献类型：
--
作者：
G. Robinson;J. Attridge;J. Deacon;S. C. Whiteley
荧光毛细管填充装置（FCFD）是一种间接光学免疫传感器，它由毛细管尺寸间隙隔开的两片玻璃组成。设备内装有完成测定所需的所有试剂，其中至少有一种是荧光标记的。一旦样品进入设备，免疫复合物，包括一定数量的荧光团标签，迅速在设备的一个表面上形成。利用用于制造该装置的玻璃片的易逝光学特性，可以从免疫角度将结合的荧光团与在该装置内保持自由（未结合）的荧光团区分开来。这允许在不需要洗涤和/或分离步骤的情况下进行分析。竞争和免疫测定都可以在FCFD内进行，并且该设备可以处理各种未经处理的样品类型（例如，全血、血清、血浆、尿液、唾液）。免疫传感器发展面临的一个主要技术挑战，即需要在传感器中加入参考/校准系统，已经得到解决，并将参考系统纳入FCFD。FCFD只需要简单的仪器来检测束缚荧光，由于其制造是基于液晶显示器的制造，因此有可能开发出大批量低成本的生产工艺。这些因素，以及设备的易用性和将参考系统纳入其中的能力，使FCFD成为分散测试的理想免疫传感器。
The fluorescent capillary fill device (FCFD) is an indirect optical immunosensor which consists of two sheets of glass held apart by a gap of capillary dimensions. Within the device are all the reagents needed to complete the assay, at least one of which is fluorescently labelled. Once the sample has entered the device, an immune complex, including an amount of the fluorophore label, rapidly forms on one of the surface of the device. Exploitation of the evanescent optical properties of the glass sheet used to fabricate the device enables the fluorophore bound immunologically to be discriminated from that which remains free (unbound) within the device. This allows the assay to be performed without the need for a wash and/or separation step. Both competitive and immunometric assays can be performed within the FCFD and the device will operate with a variety of untreated sample types (e.g., whole blood, serum, plasma, urine, saliva). A major technical challenge facing the development of immunosensors, the need to incorporate a reference/calibration system into the sensor, has been tackled and a reference system incorporated into the FCFD. The FCFD requires only simple instrumentation to interrogate bound fluorescence and, as its fabrication is based upon that of liquid-crystal displays, there is the possibility of developing a high-volume low-cost manufacturing process for its production. These factors, along with the ease of use of the device and the ability to incorporate a referencing system into it, result in the FCFD being an ideal immunosensor for decentralized testing.