Energy Expenditure is Affected by Rate of Accumulation of Sleep Deficit in Rats
Energy Expenditure is Affected by Rate of Accumulation of Sleep Deficit in Rats
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DOI：
10.1093/sleep/33.9.1226
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发表时间：
2010-09-01
期刊：
影响因子：
5.6
通讯作者：
Stephenson, Richard
中科院分区：
文献类型：
--
作者：
Caron, Aimee M.;Stephenson, Richard
研究目标：睡眠不足是肥胖的一个推定危险因素。然而，长期完全睡眠剥夺（TSD）会导致啮齿类动物能量负平衡和体重减轻，而睡眠受限的人类往往会体重增加。我们假设能量消耗（（V）超过 dotO(2)）受到大鼠睡眠不足累积率的影响。设计和干预：六只 Sprague-Dawley 大鼠在平衡交叉设计中接受慢性睡眠限制（CSR，ZT0-6 6 小时睡眠机会，持续 10 天）和刺激控制方案（CON，12 小时睡眠机会，持续 10 天，刺激数量匹配）。另外四只大鼠接受了 TSD（4 天）。使用电机驱动的步行轮来操纵睡眠。 测量和结果：通过遥测技术测量脑电图、肌电图和体温，并通过呼吸测量法测量(V) over dotO(2)。 CSR、CON 和 TSD 方案累计的总睡眠不足时间分别为 55.1 +/- 6.4 小时、31.8 +/- 6.8 小时和 38.2 +/- 2.3 小时。对 TSD 的反应证实了之前关于 dotO(2) 和体温升高 (V) 的报告。尽管累积睡眠不足较多，但这些反应在企业社会责任中减弱了。 (V) 过点的上升率与睡眠不足累积率密切相关，高于 3.6 小时·天 (-1) 的阈值不足。结论： (V) 过点的变化受睡眠不足累积率影响，而不是受总睡眠损失的影响。尽管累积的睡眠不足较多，但当大鼠每天有短暂的睡眠机会（CSR）时，在 TSD 期间观察到的负能量平衡会大大减弱。
Study Objectives: Short sleep is a putative risk factor for obesity. However, prolonged total sleep deprivation (TSD) leads to negative energy balance and weight loss in rodents, whereas sleep-restricted humans tend to gain weight. We hypothesized that energy expenditure ((V) over dotO(2)) is influenced by the rate of accumulation of sleep deficit in rats.Design and Intervention: Six Sprague-Dawley rats underwent chronic sleep-restriction (CSR, 6-h sleep opportunity at ZT0-6 for 10 days) and stimulus-control protocols (CON, 12-h sleep opportunity for 10 days, matched number of stimuli) in a balanced cross-over design. Four additional rats underwent TSD (4 days). Sleep was manipulated using a motor-driven walking wheel.Measurements and Results: Electroencephalography, electromyography, and body temperature were measured by telemetry, and (V) over dotO(2), by respirometry. Total sleep deficits of 55.1 +/- 6.4 hours, 31.8 +/- 6.8 hours, and 38.2 +/- 2.3 hours accumulated over the CSR, CON, and TSD protocols, respectively. Responses to TSD confirmed previous reports of elevated (V) over dotO(2) and body temperature. These responses were attenuated in CSR, despite a greater cumulative sleep deficit. Rate of rise of (V) over dot was strongly correlated with rate of accumulation of sleep deficit, above a threshold deficit of 3.6 h.day(-1).Conclusion: The change in (V) over dot is affected by rate of accumulation of sleep deficit and not the total sleep loss accrued. Negative energy balance, observed during TSD, is strongly attenuated when brief daily sleep opportunities are available to rats (CSR), despite greater accumulated sleep deficit.