Transdifferentiation of rat hepatic stellate cells results in leptin expression
Transdifferentiation of rat hepatic stellate cells results in leptin expression
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DOI：
10.1006/bbrc.1997.8193
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发表时间：
1998-03-06
影响因子：
3.1
通讯作者：
Anania, FA
中科院分区：
文献类型：
--
作者：
Potter, JJ;Womack, L;Anania, FA
瘦素是一种肽激素，对调节脂肪代谢至关重要。最近，据报道，酒精性肝硬化患者的循环瘦素水平较高。在健康状态下，肝星状细胞储存类视黄醇，但肝损伤后，它们转分化为肌成纤维细胞样细胞，并损失类视黄醇储存。通过 RT-PCR 分析检测瘦素 mRNA，并通过共聚焦显微镜观察培养 14 天或更长时间的转分化星状细胞中的免疫组织化学，证明瘦素表达。在新鲜分离的静止星状细胞中未发现瘦素表达。在新鲜分离或培养的库普弗细胞中未证实瘦素表达。用1μM视黄酸或10μM视黄醇乙酸酯处理活化的星状细胞导致瘦素mRNA表达的抑制。培养物中活化的星状细胞可以表达瘦素的观察结果对于了解肝病中的脂肪细胞生物学和肝硬化营养不良的治疗具有重要意义。 (C) 1998 年学术出版社。
Leptin is a peptide hormone that appears critical in regulating Fat metabolism. Recently, circulating leptin levels were reported higher in patients with alcoholic cirrhosis. In health, hepatic stellate cells store retinoids, but following Liver injury they transdifferentiate into myofibroblast-like cells with loss of the retinoid stores. Leptin expression was demonstrated by detection of leptin mRNA by RT-PCR analysis and by immunohistochemistry viewed with confocal microscopy in transdifferentiated stellate cells after 14 days, or more, of culture. Leptin expression was not found in freshly isolated quiescent stellate cells. Leptin expression was not demonstrated in freshly isolated or cultured Kupffer cells. Treatment of activated stellate cells with either 1 mu M retionic acid or 10 mu M retinol acetate resulted in the inhibition of leptin mRNA expression. The observation that activated stellate cells in culture can express leptin has implications for understanding adipocyte biology in liver disease and treatment of malnutrition in cirrhotics. (C) 1998 Academic Press.