LOCOMOTION OF AMOEBA PROTEUS
LOCOMOTION OF AMOEBA PROTEUS
复制标题
DOI：
10.1038/198675a0
复制
发表时间：
1963-01-01
期刊：
影响因子：
64.8
通讯作者：
JEON, KW
中科院分区：
文献类型：
--
作者：
BELL, LGE;JEON, KW
细胞生物学的一个中心问题是细胞的运动和运动。许多工作者研究变形虫的运动，并提出了几种机制来解释这些现象1 -3。我们得出的结论是，一个令人满意的变形虫运动的解释必须适应Dellinger多年前首次进行的观察。在我们研究变形虫运动控制的过程中，我们已经清楚地看到，在盖玻片下、毛细管中或浅玻璃室中以通常的方式检查变形虫，会给出非常不完整的情况。我们已经重新调查的序列中的事件的运动使用德林杰的方法观察变形虫从侧面和附近的变形虫没有盖玻片。总的来说，德林杰的描述得到了证实，我们的工作揭示了更多的事实。我们使用变形阿米巴饥饿24小时，洗涤并在清洁玻璃上的Chalkley溶液中观察。还检查了变形虫（King's College菌株）和Pelomyxa carolinensis。变形虫在玻璃室中观察（图1），可以从侧面和顶部观察，或者在带有棱镜的室中观察，可以从侧面观察变形虫（图2）。
Acentral problem in cell biology is cellular movement and locomotion. Many workers investigate the locomotion of amoebre and several mechanisms have been proposed to account for the phenomena observed1-3• We have come to the conclusion that a satisfactory account of amoeboid locomotion must accommodate observations first made by Dellinger• many years ago. It has become clear during our work on the control of amoeboid locomotion that to examine an amoebre in the usual way under a coverslip, in a capillary tube, or a shallow glass chamber gives a very incomplete picture of the situation. We have re-investigated the sequence of events in locomotion using Dellinger's method of observing amoebre from the side and without a coverslip near to the amoebre. In general, Dellinger's description is confirmed and our work has revealed additional facts. We have used Amoeba proteus starved for 24 h, washed and observed in Chalkley's solution on clean glass. Amoeba dubia (King's College strain) and Pelomyxa carolinensis were also examined. The amoebre were observed either in a glass chamber,(Fig. 1), which allowed observation from the side and the top, or in a chamber with a prism which allowed a side view of the amoebre,(Fig. 2).