Physiological coupling of donor and host cardiomyocytes after cellular transplantation
Physiological coupling of donor and host cardiomyocytes after cellular transplantation
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DOI：
10.1161/01.res.0000075089.39335.8c
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发表时间：
2003-06-13
影响因子：
20.1
通讯作者：
Field, LJ
中科院分区：
文献类型：
--
作者：
Rubart, M;Pasumarthi, KBS;Field, LJ
细胞移植已成为治疗心脏疾病的一种潜在方法。虽然细胞移植可以影响整体心脏功能，但目前尚不清楚这是通过供体心肌细胞的功能整合直接导致的，还是通过增强血管重建和/或改变损伤后重塑间接导致的。为了确定供体心肌细胞与宿主心肌功能整合的程度，将表达增强绿色荧光蛋白的转基因胎儿心肌细胞移植到非转基因成年小鼠的心脏中。然后使用双光子分子激发激光扫描显微镜同时成像完整受体心脏内供体细胞和宿主细胞中的细胞钙瞬变。供体心肌细胞的钙瞬变与邻近宿主心肌细胞的钙瞬变是同步的，且动力学上没有区别。这些结果有力地表明，供体心肌细胞与宿主心肌细胞在功能上偶联，并支持移植心肌细胞可以与宿主心肌形成功能性合胞的观点。
Cellular transplantation has emerged as a potential approach to treat diseased hearts. Although cell transplantation can affect global heart function, it is not known if this results directly via functional integration of donor myocytes or indirectly via enhanced revascularization and/or altered postinjury remodeling. To determine the degree to which donor cardiomyocytes are able to functionally integrate with the host myocardium, fetal transgenic cardiomyocytes expressing enhanced green fluorescent protein were transplanted into the hearts of nontransgenic adult mice. Two-photon molecular excitation laser scanning microscopy was then used to simultaneously image cellular calcium transients in donor and host cells within the intact recipient hearts. Calcium transients in the donor cardiomyocytes were synchronous with and had kinetics indistinguishable from those of neighboring host cardiomyocytes. These results strongly suggest that donor cardiomyocytes functionally couple with host cardiomyocytes and support the notion that transplanted cardiomyocytes can form a functional syncytium with the host myocardium.