PIXE analysis of human brain tissue
PIXE analysis of human brain tissue
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DOI：
10.1002/xrs.484
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发表时间：
2001-05-01
影响因子：
1.2
通讯作者：
Korman, A
中科院分区：
文献类型：
--
作者：
Boruchowska, M;Lankosz, M;Korman, A
质子激发X射线荧光分析（PIXE）技术用于少量医学样品中微量元素的定位分析。研究了人脑的白色和灰质。尸检时从17至88岁的患者中采集组织样本。进行了定性和定量分析。NIST SRM 1577 b牛肝和SRM 1566 a牡蛎组织用作测定脑标本中微量元素质量分数的外标物。这些初步研究的主要目标是评估使用PIXE对医学材料进行定量分析的方法，同时考虑到患者年龄对元素积累和元素在人脑中的区域分布的影响。计算选定元素的检测限和定量限。观察到人脑白色膜锌浓度随年龄增长而升高。在所有情况下，S、K、Ca、Mn、Fe、Cu和Zn等元素在灰质中的质量分数明显高于白色物质。本研究证实PIXE是一种灵敏度高的组织样品多元素分析技术。版权所有（C）2001约翰威利父子有限公司
The proton induced x-ray emission (PIXE) technique was used for localized analysis of trace elements in small quantities of medical samples. Both white and gray matter of the human brain were studied. The tissue samples were taken at autopsy from patients aged from 17 to 88 years. Qualitative and quantitative analyses were performed. NIST SRM 1577b Bovine Liver and SRM 1566a Oyster Tissue were used as external standards for the determination of trace element mass fractions in the brain specimens. The main goal of these preliminary studies was to evaluate approaches for the quantitative analysis of medical materials using PIXE taking into account the effect of patient age on the accumulation of elements and the regional distribution of elements in human brain. Detection and quantification limits were calculated for selected elements. Elevation of Zn concentration with age of patients was observed for white mater of the human brain. Elements such as S, K, Ca, Mn, Fe, Cu and Zn clearly exhibit higher mass fractions in grey matter than in white matter in all cases. This investigation confirms that PIXE is a useful technique for multielement analysis of tissue samples with good sensitivity. Copyright (C) 2001 John Wiley & Sons, Ltd.