Crystal structure of mouse CD1d bound to the self ligand phosphatidylcholine:: A molecular basis for NKT cell activation
Crystal structure of mouse CD1d bound to the self ligand phosphatidylcholine:: A molecular basis for NKT cell activation
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DOI：
10.4049/jimmunol.175.2.977
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发表时间：
2005-07-15
影响因子：
4.4
通讯作者：
Degano, M
中科院分区：
文献类型：
--
作者：
Giabbai, B;Sidobre, SP;Degano, M
NKT 细胞是免疫调节淋巴细胞，其激活是通过 TCR 识别 CD1d 分子中的脂质 Ag 来触发的。在本研究中，我们展示了与磷脂酰胆碱结合的小鼠 CD1d 的 2.8 埃晶体结构。配体酰基链和 CD1d 分子之间的相互作用定义了脂质 Ag 与 CD1d 结合的结构和化学要求。极性头基朝向 α 1 螺旋 C 末端的方向为在不变 NKT 细胞中观察到的 V α 链偏向提供了结构基础的基本原理。配体对蛋白质表面的贡献表明 NKT 细胞 TCR 识别脂质 Ag 的可能模式。
NKT cells are immunoregulatory lymphocytes whose activation is triggered by the recognition of lipid Ags in the context of the CD1d molecules by the TCR. In this study we present the crystal structure to 2.8 angstrom of mouse CD1d bound to phosphatidylcholine. The interactions between the ligand acyl chains and the CD1d molecule define the structural and chemical requirements for the binding of lipid Ags to CD1d. The orientation of the polar headgroup toward the C terminus of the alpha 1 helix provides a rationale for the structural basis for the observed V alpha chain bias in invariant NKT cells. The contribution of the ligand to the protein surface suggests a likely mode of recognition of lipid Ags by the NKT cell TCR.