Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
复制标题
DOI：
10.1016/j.ab.2020.113812
复制
发表时间：
2020-09-15
影响因子：
2.9
通讯作者：
Kurihara, Hidemi
中科院分区：
文献类型：
--
作者：
Kitagawa, Masae;Nagamine, Kentaro;Kurihara, Hidemi
本研究研究了一种使用环介导等温扩增 (LAMP) 快速检测与脑微出血相关的 cnm 阳性变形链球菌 (S. mutans) 的方法。 LAMP 扩增了 cnm 基因质粒载体，但不扩增人类或微生物基因组 DNA。培养1天后，无需提取DNA即可在唾液中检测到cnm阳性变形链球菌菌株的cnm DNA。该方法检测102份样本，阳性率为26.4%，高于常规PCR检测结果。总之，LAMP可用于大量样品中cnm阳性变形链球菌的检测。
This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnmpositive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.