Transgenesis in Xenopus using the Sleeping Beauty transposon system.
Transgenesis in Xenopus using the Sleeping Beauty transposon system.
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使用睡美人转座子系统对非洲爪蟾进行转基因。
DOI：
10.1002/dvdy.21994
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Mead,PaulE
中科院分区：
文献类型：
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作者：
Yergeau,DonaldA;JohnsonHamlet,MichelleR;Kuliyev,Emin;Zhu,Haiqing;Doherty,JoanneR;Archer,TaylorD;Subhawong,AndreaP;Valentine,MarcB;Kelley,ClairM;Mead,PaulE
基于转座子的整合系统已广泛用于无脊椎动物和植物模型系统的遗传操作。在过去的十年中，这些强大的工具已经开始被用于脊椎动物的转基因，插入诱变，和基因治疗的应用。睡美人（SB）是一个成员的Tc 1/水手类转座酶，是从大西洋鲑鱼分离的基因的失活形式。SB已被广泛用于人类细胞系和整个动物脊椎动物模型系统，如小鼠，大鼠和斑马鱼。在本研究中，我们描述了SB在二倍体热带爪蟾中的应用，以获得稳定的转基因品系。热带基因组通过一个非典型的过程，并通过生殖系。我们比较了SB在该模式生物中的活性与Tol 2，ahAT（流浪汉，Ac 1，TAM）样转座子系统的活性。发展动力学238：1727-1743，2009年。© 2009 Wiley利斯公司
Transposon‐based integration systems have been widely used for genetic manipulation of invertebrate and plant model systems. In the past decade, these powerful tools have begun to be used in vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications.Sleeping Beauty(SB) is a member ofTc1/marinerclass of transposases and is derived from an inactive form of the gene isolated from Atlantic salmon.SBhas been used extensively in human cell lines and in whole animal vertebrate model systems such as the mouse, rat, and zebrafish. In this study, we describe the use ofSBin the diploid frogXenopus tropicalisto generate stable transgenic lines.SBtransposon transgenes integrate into theX. tropicalisgenome by a noncanonical process and are passed through the germline. We compare the activity ofSBin this model organism with that ofTol2, ahAT (hobo, Ac1, TAM)‐like transposon system. Developmental Dynamics 238:1727–1743, 2009. © 2009 Wiley‐Liss, Inc.