Androgens modulate interleukin-6 production by gingival fibroblasts in vitro
Androgens modulate interleukin-6 production by gingival fibroblasts in vitro
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DOI：
10.1902/jop.1999.70.6.604
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发表时间：
1999-06-01
影响因子：
4.3
通讯作者：
Zamorano, P
中科院分区：
文献类型：
--
作者：
Gornstein, RA;Lapp, CA;Zamorano, P
背景：妊娠期和青春期牙周炎被归因于循环性激素浓度的增加。这种牙周炎性疾病伴随着局部细胞因子的产生。本研究的目的是在体外研究睾酮(T)和双氢睾酮(DHT)对人牙龈成纤维细胞(HGF)分泌白介素6(IL-6)的影响，以及常用的抗雄激素药物氟他胺对HGF分泌IL-6的影响。细胞在有或没有雄激素的情况下孵育72天。用放射免疫法测定IL-1β加激素刺激24小时后分泌到培养液中的IL-6浓度。结果：在无血清条件下，5×10~(-8)~10~(-7)M的T和DHT显著抑制HGF产生IL-6(P<；0.05)，2×10~(-5)M的T和DHT不能逆转这种抑制作用。结论：雄激素水平的升高，尤其是睾酮和双氢睾酮水平的升高，可能通过下调IL-6的产生而影响基质细胞对炎症刺激的反应。这项体外研究支持了一种假设，即怀孕或青春期激素增加可以调节局部炎症的发展。
Background: Pregnancy and puberty gingivitis have been attributed to increased concentrations of circulating sex hormones. This inflammatory gingival condition is accompanied by the local production of cytokines. The aims of this in vitro study were to assess, in the presence or absence of testosterone (T) or dihydrotestosterone (DHT), the production of interleukin-6 (IL-6) by human gingival fibroblasts (hGF), and to evaluate the effects of flutamide (a common anti-androgen) in this system.Methods: The effects of the androgens, T and DHT, on IL-6 production were measured in vitro in serum-free, phenol red-free medium. Cells were incubated with or without androgens for 72. hours; the concentration of IL-6 secreted into the medium after an additional 24-hour challenge with IL-1 beta plus hormones was estimated by radioimmunoassay. The reverse transcription polymerase chain reaction was used to examine hGF and periodontal ligament cells (PDL) for the presence of androgen receptor.Results: In serum-free medium, T and DHT at concentrations of 5 x 10(-8) to 10(-7)M significantly (P < 0.05) inhibited IL-6 production by hGF Flutamide, up to concentrations of 2 x 10(-5)M, did not reverse this inhibition. The androgen receptor was identified in both hGF and PDL.Conclusions: We concluded that elevated levels of androgens, specifically testosterone and dihydrotestosterone, could affect the stromal cell response to an inflammatory challenge by downregulation of IL-6 production. This in vitro study lends support to the hypothesis that increased hormones during pregnancy or puberty could modulate the development of localized inflammation.