The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model
The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model
复制标题
定量肝脏实验模型中细粒棘球绦虫生长过程中的局部免疫反应
DOI：
10.1038/s41598-019-56098-3
复制
发表时间：
2019-12-23
影响因子：
4.6
通讯作者：
Wen, Hao
中科院分区：
文献类型：
--
作者：
Li, Zhide;Zhang, Chuanshan;Wen, Hao
细粒棘球蚴病在肝脏中发生发展的局部免疫机制尚不清楚。我们开发了一个合适的实验模型，模仿自然感染的肝脏使用门静脉注射的protoeces。与剂量依赖性的多房棘球蚴感染相反，无论注射剂量如何，完全成熟的包虫囊肿都可以在肝脏中建立;尽管大多数感染部位在建立阶段被视为与纤维化相关的炎性肉芽肿，但它们从未成熟为囊肿。在建立阶段，强烈的免疫应答由T和B细胞组成，肝脏中有T1型、T2型细胞和细胞因子以及分泌IL-10的CD 8 +T细胞。在建立的阶段，结果表明由B细胞局部产生抗体，并且涉及NK和NKT细胞。感染的结果和局部免疫反应在肝脏，是不同的细粒棘球绦虫狭义和多房棘球绦虫的小鼠模型分别，然而，只有在微环境水平的早期特异性可能解释的主要差异发现的病变由两个物种。我们的定量实验模型似乎完全适合于进一步研究这种微环境及其与每个绦虫物种的关系。
The local immune mechanisms responsible for the establishment and development ofEchinococcus granulosus sensu strictoinfection in the liver, have been little explored. We developed a suitable experimental model that mimics naturally infected livers using portal injection of protoscoleces. Opposite toEchinococcus multilocularisinfection which is dose-dependent, fully mature hydatid cysts can be established in the liver whatever the injection dose; although most of the infection sites were seen at the establishment phase as inflammatory granulomas associated with fibrosis, they never matured into cysts. At the establishment phase, a strong immune response was composed of T and B cells, with T1-type, T2-type cells and cytokines and IL-10-secreting CD8+T cells in the liver. At the established phase, results suggested a local production of antibodies by B cells, and an involvement of NK and NKT cells. Infection outcome and local immune response in the liver, were different in the mouse models ofEchinococcus granulosus sensu strictoandEchinococcus multilocularisrespectively; however, only early specificities at the microenvironment level might explain the major differences found between the lesions induced by the two species. Our quantitative experimental model appears fully appropriate to further study this microenvironment and its relationship with each cestode species.