Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells
Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells
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DOI：
10.1111/j.1348-0421.2004.tb03614.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Song, K
中科院分区：
文献类型：
--
作者：
Kee, SH;Cho, EJ;Song, K
风疹病毒（rubella virus，RV）的感染性侵入是通过受体介导的内吞作用进行的。为探讨RV的细胞内吞机制，分析了影响各种内吞途径的药物对RV进入VeroE6细胞的抑制作用。结果表明，RV感染进入VeroE6细胞是由网格蛋白依赖的内吞作用介导的，而不是由小窝介导的内吞作用。此外，化学抑制巨胞饮作用，如阿米洛利，肌动蛋白和微管破坏药物的治疗显着减少RV感染。考虑到巨胞饮是由细胞刺激诱导的内吞作用，网格蛋白介导的内吞作用可能是RV感染进入的主要途径。
It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into VeroE6 cells is mediated by clathrin-dependent endocytosis and not by caveolae-mediated endocytosis. Moreover, chemical inhibition of macropinocytosis such as treatments of amiloride, actin and microtubule-disrupting drug significantly reduced RV infection. Considering that macropinocytosis is inducible endocytosis by cellular stimulations, clathrin-mediated endocytosis is likely to be a major route of RV infectious entry.