Detection of virus antigens in Swiss albino mice infected by milk-borne mouse mammary tumour virus: the effect of age, sex and reproductive status. II. Radioimmunoassay of two virus components, gp47 and p28 in serum and organ extracts.
Detection of virus antigens in Swiss albino mice infected by milk-borne mouse mammary tumour virus: the effect of age, sex and reproductive status. II. Radioimmunoassay of two virus components, gp47 and p28 in serum and organ extracts.
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感染乳源性小鼠乳腺肿瘤病毒的瑞士白化小鼠中病毒抗原的检测：年龄、性别和生殖状态的影响。
DOI：
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发表时间：
1979
影响因子：
3.8
通讯作者：
L. Gosselin
中科院分区：
文献类型：
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作者：
P. Osterrieth;S. Kozma;J. Hendrick;C. François;C. Calberg‐Bacq;P. Franchimont;L. Gosselin
用放射免疫法检测了不同年龄、性别和生理状况的乳源性MMTV感染瑞士白化病小鼠的各种器官、乳腺肿瘤和血清提取物中gp47（主要包膜多肽）和p28（病毒主要核心蛋白）的存在。除了在大脑、卵巢和睾丸中，这两种抗原在老年动物和首次怀孕后的雌性动物的所有器官中都有发现。抗原不存在于断奶的器官或整个胎儿。在乳腺、乳腺肿瘤、附睾和精囊中发现较高的值。这些器官也含有比p28更多的gp47。血清中一般含有gp47，很少含有p28。这表明gp47在血液中不与病毒粒子结合。妊娠、哺乳期，特别是乳腺肿瘤的存在使血清中gp47的浓度升高。结果不允许在哺乳小鼠摄入病毒和第一次怀孕之间的间隔中定位MMTV感染的靶器官。此外，在一组缺乏外源性病毒的小鼠中获得的结果表明，由于内源性MMTV基因组表达p28，它可能解释了部分检测到外源性MMTV感染小鼠的p28。
Extracts of various organs, mammary tumours and sera from milk-borne MMTV infected Swiss albino mice of different age, sex and physiological conditions were tested by radioimmunoassay for the presence of gp47, the main envelope polypeptide, and p28, the main core protein of the virus. Except in brain, ovaries and testes, both antigens were found in all organs of old animals and of females after the onset of their first pregnancy. Antigens were not present in organs of weanlings or in whole foetuses. Higher values were found in mammary glands, mammary tumours, epididymis and seminal vesicles. These organs also harboured a greater amount of gp47 than p28. The serum generally contained gp47 but rarely p28. This indicates that gp47 is not virion-bound in blood. Pregnancy, lactation and especially the presence of mammary tumours increased the concentration of gp47 in serum. The results do not allow localization of target organs of MMTV infection in the interval between ingestion of the virus by the suckling mouse and the first pregnancy. Moreover, results obtained with one group of mice devoid of exogenous virus show that, as endogenous MMTV genome expresses p28, it might account for part of the p28 detected exogenous MMTV-infected mice.