belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain
belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain
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DOI：
10.1242/dev.02244
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发表时间：
2006-02-01
期刊：
影响因子：
4.6
通讯作者：
Karlstrom, RO
中科院分区：
文献类型：
--
作者：
Seth, A;Culverwell, J;Karlstrom, RO
在脊椎动物前脑中形成的一些最早的轴突通路是在连合和视网膜轴突穿过间脑和端脑的中线时建立的。为了更好地理解前脑中的轴突导向，我们描述了斑马鱼颠茄（bel）突变，它破坏了前脑中的连合和视网膜轴突导向。使用定位克隆策略，我们确定了贝尔基因座编码斑马鱼Lhx2，一个利姆同源结构域转录因子表达在大脑，眼睛和鳍芽。我们发现，贝尔（lhx2）功能是必需的图案在腹前脑和眼睛，贝尔功能的损失导致调节基因表达的改变，轴突导向因子的扰动，以及缺乏视交叉和前脑连合。我们的分析揭示了lhx2在中线轴突引导、前脑模式和眼形态发生中的新作用。
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross the midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna (bel) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of bel function leads to alterations in regulatory gene expression, perturbations in axon guidance factors, and the absence of an optic chiasm and forebrain commissures. Our analysis reveals new roles for lhx2 in midline axon guidance, forebrain patterning and eye morphogenesis.