Expression and localization of versican during postnatal development of rat temporomandibular joint disc
Expression and localization of versican during postnatal development of rat temporomandibular joint disc
复制标题
DOI：
10.1007/s00418-005-0020-1
复制
发表时间：
2006-03
影响因子：
2.3
通讯作者：
N. Toriya;T. Takuma;T. Arakawa;Y. Abiko;Y. Sasano;I. Takahashi;Y. Sakakura;F. Rahemtulla;I. Mizog
中科院分区：
文献类型：
--
作者：
N. Toriya;T. Takuma;T. Arakawa;Y. Abiko;Y. Sasano;I. Takahashi;Y. Sakakura;F. Rahemtulla;I. Mizog
为探讨颞下颌关节（TMJ）盘细胞外基质成分的生长相关变化，采用Western blot、实时定量PCR和免疫组织化学方法检测了大鼠生后（2-32周）TMJ盘中硫酸软骨素蛋白聚糖（versican）核心蛋白的表达和定位。Western blot分析显示，大鼠颞下颌关节盘主要表达一种亚型（V1），其核心蛋白在出生后急剧增加，8周时达到高峰，32周后逐渐下降。TaqMan探针实时定量PCR结果显示，versican mRNA表达量在2周时最高，随着生长逐渐下降。免疫组织化学研究显示，多功能蛋白聚糖染色弱，均匀分布在颞下颌关节盘在2周。多功能蛋白聚糖染色的区域差异在8周后变得突出;染色在前部和后部外周附件中是强烈的，并且在椎间盘的中央部分是弱的。这些结果表明，生长期大鼠颞下颌关节盘中versican的表达存在生长相关性变化和区域性差异，可能反映了口面功能发育过程中生物力学环境的变化。
To analyze the growth-related changes in extracellular matrix components in temporomandibular joint (TMJ) discs, the expression and localization of the core protein of a large chondroitin sulphate proteoglycan, versican, in rat TMJ discs during postnatal development (2–32 weeks) were examined using Western blot analysis, real-time quantitative PCR and immunohistochemistry. Western blot analysis showed that rat TMJ discs predominantly expressed one isoform (V1) and the core protein sharply increased after birth, reached a peak at 8 weeks, and then gradually decreased up to 32 weeks. Real-time quantitative PCR with TaqMan probes indicated that mRNA expression of versican was highest at 2 weeks and gradually decreased with growth. An immunohistochemical study showed that staining for versican was weak and evenly distributed in TMJ discs at 2 weeks. Regional differences in staining for versican became prominent after 8 weeks; staining was intense in the anterior and posterior peripheral attachments, and weak in the central part of the discs. These results demonstrate that growth-related changes and regional differences exist in the expression of versican in the TMJ discs of growing rats, and these probably reflect the changes in the biomechanical environment caused by the development of orofacial functions.