Solid-phase microextraction for the analysis of human breath
Solid-phase microextraction for the analysis of human breath
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DOI：
10.1021/ac960749l
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发表时间：
1997-02-15
影响因子：
7.4
通讯作者：
Pawliszyn, J
中科院分区：
文献类型：
--
作者：
Grote, C;Pawliszyn, J
固相微萃取（SPRaE）已被应用于定量测定乙醇，丙酮和异戊二烯在人体呼吸。该方法包括提取和预浓缩与熔融石英光纤涂有聚合物固定相，解吸在200摄氏度，并通过气相色谱/质谱分析，三种不同的纤维涂层进行了评价方面的灵敏度，线性范围，精密度和检测限。根据光纤涂层和所研究的化合物，可以获得2%-6%范围内的典型RSD值。化合物的校准曲线在人呼吸样品中发现的浓度范围内是可再现的和线性的。该方法能够检测健康受试者报告的丙酮和异戊二烯浓度。详细研究了温度和湿度对提取过程的影响。log K与1/T之间的线性关系允许在任何给定温度下校准该方法。该装置便携、经济且易于用于患者采样。
Solid-phase microextraction (SPRaE) has been applied to the quantitative determination of ethanol, acetone, and isoprene in human breath. The method involves extraction and preconcentration with a fused silica fiber coated with a polymeric stationary phase, desorption at 200 degrees C, and assay by gas chromatography/mass spectrometry, Three different fiber coatings have been evaluated with regard to sensitivity, linear range, precision, and detection limits. Typical RSD values in the range 2%-6% could be obtained, depending on the fiber coating and the compound investigated. The calibration curves for the compounds are reproducible and linear over the concentration ranges found in human breath samples. The method is capable of detecting concentrations of acetone and isoprene reported for healthy subjects. The influence of temperature and humidity on the extraction process has been studied in detail, A linear relationship between log K versus 1/T allows the calibration of the method for any given temperature, The device is portable, economical, and easy to use in patient sampling.