Association between polymorphism of human oxoguanine glycosylase 1 and risk of prostate cancer
Association between polymorphism of human oxoguanine glycosylase 1 and risk of prostate cancer
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DOI：
10.1097/01.ju.0000087498.23008.bb
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发表时间：
2003-12-01
影响因子：
6.6
通讯作者：
Park, J
中科院分区：
文献类型：
--
作者：
Chen, L;Elahi, A;Park, J
目的：人氧鸟嘌呤糖基酶1 （hOGG1）基因编码一种DNA糖基酶，参与从氧化损伤的DNA中切除修复8-OH-dG（8-羟基-2-脱氧鸟嘌呤）。为了确定hOGG1是否在前列腺癌风险中起作用，我们筛选了正常前列腺组织标本中hOGG1的表达，并评估了hOGG1 Ser326Cys多态性在前列腺癌风险中的作用。材料与方法：采用逆转录-聚合酶链反应法检测5例正常前列腺组织中hOGG1的表达。采用聚合酶链反应限制性片段长度多态性分析方法，对84例原发性前列腺癌患者和252例白人男性个体匹配对照（按年龄（诊断时±5岁）按1:3比例）分离的基因组DNA进行分析，比较白人患者和对照组之间hOGG1 Ser326Cys多态性的流行程度。结果：在所检测的前列腺组织中均检测到hOGG1 mRNA。hOGG1基因型与前列腺癌呈剂量效应关系(趋势检验p
Purpose: The human oxoguanine glycosylase 1 (hOGG1) gene encodes a DNA glycosylase that is involved in excision repair of 8-OH-dG (8-hydroxy-2-deoxyguanine) from oxidatively damaged DNA. To determine whether hOGG1 has a role in the risk of prostate cancer we screened normal prostate tissue specimens for hOGG1 expression and assessed the role of hOGG1 Ser326Cys polymorphism in the risk of prostate cancer.Materials and Methods: In 5 normal prostate tissues hOGG1 expression was determined by reverse transcriptase-polymerase chain reaction. The prevalence of hOGG1 Ser326Cys polymorphism was compared between white patients cases and controls using polymerase chain reaction restriction fragment length polymorphism analysis of genomic DNA isolated from 84 incident patients with primary prostate cancer and 252 individually matched controls (1:3 ratio) by age (+/- 5 years at diagnosis) in white men.Results: In all prostate tissues tested hOGG1 mRNA was detected. A significant association was found between hOGG1 genotypes and prostate cancer with a dose effect relationship (trend test p