Rift Valley fever virus noncoding regions of L, M and S segments regulate RNA synthesis
Rift Valley fever virus noncoding regions of L, M and S segments regulate RNA synthesis
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DOI：
10.1016/j.virol.2006.03.018
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发表时间：
2006-07-20
期刊：
影响因子：
3.7
通讯作者：
Bouloy, Michele
中科院分区：
文献类型：
--
作者：
Gauliard, Nicolas;Billecocq, Agnes;Bouloy, Michele
裂谷热病毒（裂谷热病毒）（布尼亚病毒科白蛉病毒）具有由三个负链RNA分子组成的基因组。每个片段包含3‘和5’非编码区，末端互补序列形成一个长柄结构。我们发现L、M和S片段的转录复制受到调控，我们建立了一个表达CAT报告子的小基因组拯救系统来研究非编码区在这一过程中的作用。基于L、M和S片段的小基因组被证明可以驱动真正的转录和复制，并表达不同水平的CAT报告子，这表明在非编码序列中启动子的活性是不同的。此外，我们发现rvfv感染细胞中启动子的相对强度与病毒RNA种类的丰度之间存在良好的相关性。总之，这些结果表明，RVFV小基因组是研究转录和复制的有力工具，并构成了从cdna中拯救感染性病毒的宝贵基础。(c) 2006爱思唯尔公司版权所有。
Rift Valley fever virus (RVFV) (Phlebovirus, Bunyaviridae) possesses a genome composed of three negative-stranded RNA molecules. Each segment contains 3' and 5' noncoding regions with terminal complementary sequences forming a panhandle structure. We showed that transcription-replication of the L, M and S segments is regulated, and we established a minigenome rescue system expressing a CAT reporter to investigate the role of the noncoding regions in this process. The L, M and S segment-based minigenomes were shown to drive bona fide transcription and replication and to express variable levels of CAT reporter, indicating differential promoter activities within the noncoding sequences. In addition, we found a good correlation between the relative promoter strength and the abundance of viral RNA species in RVFV-infected cells. Altogether, these results show that RVFV minigenomes are powerful tools to study transcription and replication and constitute a valuable basis to rescue infectious virus from cDNAs. (c) 2006 Elsevier Inc. All rights reserved.