Fabrication of aptamer modified TiO2 nanofibers for specific capture of circulating tumor cells
Fabrication of aptamer modified TiO2 nanofibers for specific capture of circulating tumor cells
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适配体修饰二氧化钛纳米纤维的制备用于特异性捕获循环肿瘤细胞
DOI：
10.1016/j.colsurfb.2020.110985
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Pei Renjun
中科院分区：
文献类型：
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作者：
Liu Hui;Sun Na;Ding Pi;Chen Changchong;Wu Zeen;Zhu Weipei;Liu Lifen;Wang Zhili;Pei Renjun
在此，我们开发了一种廉价的二氧化钛 (TiO2) 纳米纤维基材，用于从模拟患者的样本中高效、选择性地捕获循环肿瘤细胞 (CTC)。 TiO2 纳米纤维基材是通过静电纺丝结合煅烧工艺制备的。用抗粘附分子牛血清白蛋白(BSA)和核仁素适体AS1411修饰纳米纤维基材的表面，其中适体AS1411特异性结合癌细胞膜表面过表达的核仁蛋白。形成的 TiO2 纳米纤维基材通过协同拓扑相互作用表现出高效和特异性来捕获核仁素阳性细胞。利用稀有数量的细胞捕获实验，在纳米纤维基材表面上对 1mL 全血样本中白细胞 (WBC) 中掺入的稀有数量的目标细胞实现了高达 75% 的捕获效率。总之，本研究强调了 TiO2-BSA-生物素-AS1411 纳米纤维基材作为高效平台的潜力，可在临床环境中实现稀有 CTC 的选择性和特异性捕获。
Herein, we developed an inexpensive titanium dioxide (TiO2) nanofiber substrate for efficient and selective capture of circulating tumor cells (CTCs) from mimic patients’ samples. The TiO2nanofiber substrates were fabricated by electrospinning in combination with the calcination process. The surface of nanofiber substrates was modified with the anti-adhesion molecule, bovine serum albumin (BSA) and the nucleolin aptamer AS1411, wherein, aptamer AS1411 specifically binds to the nucleolin protein overexpressed on the membrane surface of cancer cells. The formed TiO2nanofiber substrates exhibited high efficacy and specificity to capture nucleolin positive cells through synergistic topographic interactions. Using the rare number of cell capture experiments, the capture efficiency of up to 75 % was achieved on the surface of the nanofiber substrate for rare number target cells spiked in the white blood cells (WBCs) from 1 mL whole blood samples. In conclusion, this study highlighted the potential of the TiO2-BSA-biotin-AS1411 nanofiber substrate as a highly efficient platform to realize the selective and specific capture of rare CTCs in the clinical settings.