T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms.
T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms.
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T 细胞受体基因重排作为 T 细胞肿瘤谱系和克隆性的标记。
DOI：
10.1073/pnas.82.10.3460
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发表时间：
1985
影响因子：
11.1
通讯作者：
Dalla-Favera,R
中科院分区：
文献类型：
--
作者：
Flug,F;Pelicci,PG;Bonetti,F;Knowles2nd,DM;Dalla-Favera,R
Ig基因重排代表了B细胞的谱系、克隆性和分化的标记，允许B细胞肿瘤的分子诊断和免疫基因型分类。我们试图通过分析T细胞受体β链（T β）基因的重排，将类似的方法应用于T细胞群体的研究。我们的分析，使用来自多克隆T细胞和12个T细胞肿瘤的T β特异性探针进行Southern blotting杂交，表明T β基因重排模式可以用作(i)谱系标记，允许鉴定多克隆T细胞群体，以及（ii）克隆性标记，允许检测单克隆T细胞肿瘤。此外，我们的数据表明，T β基因重排代表了T细胞分化的早期和一般标记，因为它们在T细胞发育的所有阶段的组织学不同的肿瘤中都可以检测到。确定谱系、克隆性和分化阶段的能力对未来正常和肿瘤T细胞的实验和临床研究具有重要意义。
Ig gene rearrangements represent markers of lineage, clonality, and differentiation of B cells, allowing a molecular diagnosis and immunogenotypic classification of B-cell neoplasms. We sought to apply a similar approach to the study of T-cell populations by analyzing rearrangements of the T-cell receptor beta-chain (T beta) gene. Our analysis, by Southern blotting hybridization using T beta-specific probes of DNAs from polyclonal T cells and from 12 T-cell tumors, indicates that T beta gene rearrangement patterns can be used as markers of (i) lineage, allowing the identification of polyclonal T-cell populations, and (ii) clonality, allowing the detection of monoclonal T-cell tumors. In addition, our data indicate that T beta gene rearrangements represent early and general markers of T-cell differentiation since they are detectable in histologically different tumors at all stages of T-cell development. The ability to determine lineage, clonality, and stage of differentiation has significant implications for future experimental and clinical studies on normal and neoplastic T cells.