Genetic analysis of RpL38 and RpL5, two Minute genes located in the centric heterochromatin of chromosome 2 of Drosophila melanogaster
Genetic analysis of RpL38 and RpL5, two Minute genes located in the centric heterochromatin of chromosome 2 of Drosophila melanogaster
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DOI：
10.1534/genetics.104.034124
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发表时间：
2005-02-01
期刊：
影响因子：
3.3
通讯作者：
Leevers, SJ
中科院分区：
文献类型：
--
作者：
Marygold, SJ;Coelho, CMA;Leevers, SJ
黑腹果蝇的微小突变被认为会破坏编码核糖体蛋白（RPs）的基因，从而损害核糖体功能和蛋白质合成。然而，相对较少的Minutes与不同的RP基因相关联，可能会发现更多的Minute位点。我们在筛选限制黑腹龙翼生长的因素时发现了RpL38和RpL5的点突变。在这里，我们提出了这些位点的第一个遗传特征。RpL38位于染色体臂2R的中心异染色质上，与先前鉴定的Minute， M(2)41A和l(2)41Af相同。RpL5位于2L中心的异染色质上，定义了一个新的Minute基因。这两个基因都是单倍不足的，因为杂合突变会导致典型的微小表型，即小刚毛和发育延迟。令人惊讶的是，我们发现RpL38(-)/+和RpL5(-)/+成年果蝇由于细胞大小的增加而具有异常大的翅膀，这强调了翻译调控在控制生长中的重要性。综上所述，我们的数据提供了两个以前未被表征的Minute/RP基因的新的分子和遗传信息，它们所在的异色区，以及它们的蛋白产物在控制器官生长中的作用。
The Minute mutations of Drosaphila melanogaster are thought to disrupt genes that encode ribosomal proteins (RPs) and thus impair ribosome function and protein synthesis. However, relatively few Minutes have been tied to distinct RP genes and more Minute loci are likely to be discovered. We have identified point mutations in RpL38 and RpL5 in a screen for factors limiting for growth of the D. melanogaster wing. Here, we present the first genetic characterization of these loci. RpL38 is located in the centric heterochromatin of chromosome arm 2R and is identical to a previously identified Minute, M(2)41A, and also l(2)41Af. RpL5 is located in the 2L centric heterochromatin and defines a novel Minute gene. Both genes are haplo-insufficient, as heterozygous mutations cause the classic Minute phenotypes of small bristles and delayed development. Surprisingly, we find that RpL38(-)/+ and RpL5(-)/+ adult flies have abnormally large wings as a result of increased cell size, emphasizing the importance of translational regulation in the control of growth. Taken together, our data provide new molecular and genetic information on two previously uncharacterized Minute/RP genes, the heterochromatic regions in which they reside, and the role of their protein products in the control of organ growth.