IgG antibodies against common gut bacteria are more diagnostic for Crohn's disease than IgG against mannan or flagellin
IgG antibodies against common gut bacteria are more diagnostic for Crohn's disease than IgG against mannan or flagellin
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DOI：
10.1111/j.1572-0241.2007.01577.x
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发表时间：
2008-02-01
影响因子：
9.8
通讯作者：
Florin, Timothy H. J.
中科院分区：
文献类型：
--
作者：
Adams, Rachel J.;Heazlewood, Sharise P.;Florin, Timothy H. J.
简介：面包酵母（甘露聚糖）抗体已广泛用于辅助诊断克罗恩病。最近，人们对来自球状梭菌亚门的鞭毛蛋白抗体产生了兴趣。我们假设与这些抗原的反应性是克罗恩病中针对肠道微生物群的 IgG 反应普遍增强的替代标志。 方法：我们将针对鞭毛蛋白和甘露聚糖的 IgG 抗体与两种复杂的表面抗原制剂的诊断效用进行了比较，其中一种来自普通双歧杆菌 (Bv)，另一种来自 20 多种常见的粘膜相关微生物群，一种多细菌膜制剂 (MBP)。在两个年龄和性别匹配人群的血清中测量了 IgG 抗体：120 名克罗恩病患者 (CD) 和 160 名胃肠病对照 (CON)（包括 40 名溃疡性结肠炎 (UC) 和 120 名非 IBD）患者。 结果：克罗恩病中针对所有抗原制剂的 IgG 均升高，但接受者操作特征 (ROC) 图的统计分析表明，针对复杂抗原制剂 MBP 和 Bv 的 IgG 升高与针对甘露聚糖的 IgG 相比，在区分两个群体（CD 和 CON）方面具有更好的诊断准确性（P
INTRODUCTION: Antibodies to baker's yeast (mannan) have been widely used to aid in diagnosis of Crohn's disease. Recently, there has been interest in antibodies against a flagellin from Clostridium coccoides subphylum. We hypothesized that reactivity with these antigens is a surrogate marker for a generalized increased IgG response against intestinal microbiota in Crohn's disease.METHODS: We compared the diagnostic utility of IgG antibodies against flagellin and mannan with two complex surface antigen preparations, one derived from B. vulgatus (Bv), the other from over 20 common mucosa-associated microbiota, a multibacterial membrane preparation (MBP). IgG antibodies were measured in sera from two age- and sex-matched populations: :120 Crohn's patients (CD) and 160 gastroenterology controls (CON) comprising 40 ulcerative colitis (UC) and :120 non-IBD) patients.RESULTS: IgG was elevated against all antigen preparations in Crohn's but statistical analysis of receiver operator characteristic (ROC) plots showed that IgG against the complex antigen preparations MBP and Bv had better diagnostic accuracy to distinguish the two populations (CD and CON) than IgG against mannan (P