Complement inhibition by soluble complement receptor type 1 improves microcirculation after rat liver transplantation
Complement inhibition by soluble complement receptor type 1 improves microcirculation after rat liver transplantation
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DOI：
10.1097/00007890-199809270-00005
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发表时间：
1998-09-27
期刊：
影响因子：
6.2
通讯作者：
Post, S
中科院分区：
文献类型：
--
作者：
Lehmann, TG;Koeppel, TA;Post, S
背景最近的观察提供了证据，补体参与缺血/再灌注损伤的病理生理学。在这项研究中，我们使用大鼠肝移植模型评估了补体抑制对肝脏微循环和移植物功能的影响。冷保存（威斯康星州大学溶液，4 ℃，24 h）后，在刘易斯大鼠中进行动脉化原位肝移植。8只动物在再灌注前1分钟静脉内接受生理性补体调节剂可溶性补体受体1（sCR 1）。对照组（n=8）给予林格氏液，再灌注30-100 min后在体显微镜下观察微血管灌注、白细胞粘附和枯否细胞吞噬活性。sCR 1组肝窦微血管灌注改善（87 ± 0.7% vs. 50 ± 1%; P
Background. Recent observations provide evidence that complement is involved in the pathophysiology of ischemia/reperfusion injury. In this study, we assessed the impact of complement inhibition on hepatic microcirculation and graft function using a rat model of liver transplantation.Methods. Arterialized orthotopic liver transplantation was performed in Lewis rats after cold preservation (University of Wisconsin solution, 4 degrees C, 24 h). Eight animals received the physiological complement regulator soluble complement receptor type 1 (sCR1) intravenously 1 min before reperfusion. Controls received Ringer's solution (n=8), Microvascular perfusion, leukocyte adhesion, and Kupffer cell phagocytic activity were studied 30-100 min after reperfusion by in vivo microscopy.Results. Microvascular perfusion in hepatic sinusoids was improved in the sCR1 group (87+/-0.7% vs. 50+1%; P