Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
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DOI：
10.1016/s0021-9258(18)46052-8
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发表时间：
1992-01
期刊：
影响因子：
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通讯作者：
J. Haefliger;R. Bruzzone;N. Jenkins;D. Gilbert;N. Copeland;D. Paul
中科院分区：
文献类型：
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作者：
J. Haefliger;R. Bruzzone;N. Jenkins;D. Gilbert;N. Copeland;D. Paul
我们使用低严格率杂交和简并寡核苷酸的聚合酶链式反应(PCR)扩增来鉴定大鼠连接蛋白基因家族的四个新成员。根据预测的分子质量，这些蛋白被命名为Cx40(Cx40)、Cx37、Cx33和Cx31.1。新的连接蛋白显示了连接蛋白家族的所有保守结构特征，包括高度相似的胞外和跨膜结构域，但主要的细胞质结构域却不同。根据初级序列的相似性，连接蛋白家族可分为两类。Cx40、Cx37和Cx33与先前描述的Cx43和Cx46相似。Cx31.1类似于Cx26、Cx31和Cx32。Cx37和Cx40mRNAs在各种成体器官和组织中都有表达，其中在肺中的表达尤为丰富。然而，它们在许多器官中的相对水平不同，因此它们的分布并不完全一致。Cx33和Cx31.1基因表现出更多的限制性表达模式；mRNAs分别在睾丸和皮肤中检测到。染色体定位研究表明，Cx26和Cx46紧密连锁在14号染色体上，Cx37和Cx31.1连锁在4号染色体上，其余连接蛋白基因分散分布。
We have used low stringency hybridization and polymerase chain reaction (PCR) amplification with degenerate oligonucleotides to identify four new members of the rat connexin gene family. On the basis of their predicted molecular mass, these proteins have been designated connexin (Cx) 40 (Cx40), Cx37, Cx33, and Cx31.1. The new connexins exhibit all of the conserved structural features of the connexin family, including highly similar extracellular and transmembrane domains but divergent major cytoplasmic domains. On the basis of primary sequence similarity, the connexin family may be divided into two classes. Cx40, Cx37, and Cx33 are similar to the previously characterized Cx43 and Cx46. Cx31.1 is similar to Cx26, Cx31, and Cx32. Cx37 and Cx40 mRNAs are expressed in a wide variety of adult organs and tissues, with particular abundance in lung. However, their relative levels are different in many organs and thus their distribution is not completely coincident. Cx33 and Cx31.1 genes exhibit a much more restricted pattern of expression; mRNAs are detected only in testes and skin, respectively. Chromosomal mapping studies indicate that Cx26 and Cx46 are tightly linked on chromosome 14, and Cx37 and Cx31.1 are linked on chromosome 4, while the rest of the connexin genes are dispersed.