Physiological role of the aryl hydrocarbon receptor in mouse ovary development
Physiological role of the aryl hydrocarbon receptor in mouse ovary development
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DOI：
10.1093/toxsci/56.2.382
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发表时间：
2000-08-01
影响因子：
3.8
通讯作者：
Flaws, JA
中科院分区：
文献类型：
--
作者：
Benedict, JC;Lin, TM;Flaws, JA
芳烃受体(AhR)调节2，3，7，8-四氯二苯并-对二恶英(TCDD)等环境污染物的毒性。由于AhR在卵巢中的生理作用尚不清楚，本研究的目的是验证AhR调节卵巢卵泡出现和数量的假说。取AhR基因缺陷小鼠(AhRKO)和野生型小鼠在妊娠第18天(GD18)和出生后第2~3天、第8天、32~35天和53天的卵巢。对完整的卵巢连续切片进行组织学评估，以确定生殖细胞和卵泡的存在。在GD18上，AhRKO和野生型胎儿的每个卵巢生殖细胞数没有差异。然而，在PND2-3时，AhRKO小鼠的原始卵泡的形成明显多于野生型小鼠(AhRKO=38,440+/-3632比野生型=21,120+/-2688)，单个生殖细胞比野生型小鼠少(AhRKO=12,696+/-1192比野生型=18,160+/-720)。在第8天和第32-35天，AhRKO小鼠与野生型小鼠的卵泡数无明显差异，但在PND 53，AhRKO小鼠的有腔卵泡数显著少于野生型(AhRKO=3416+/-480vs，野生型=6776+/-1024)。综上所述，这些结果提示AhR可能在原始卵泡的形成和有腔卵泡数量的调节中起作用。
The aryl hydrocarbon receptor (AhR) regulates the toxicity of environmental contaminants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). As the physiological role of the AhR in the ovary is unknown, the purpose of this study was to test the hypothesis that the AhR regulates the appearance and numbers of ovarian follicles. Ovaries were harvested from AhR-deficient (AhRKO) and wild-type mice on gestational day 18 (GD 18) and postnatal days (PND) 2-3, 8, 32-35, and 53. Complete serial sections of ovaries were evaluated histologically for the presence of germ cells and follicles. On GD 18, there was no difference in the number of germ cells per ovary between AhRKO and wild-type fetuses. However, by PND 2-3, AhRKO mice had significantly more fully formed primordial follicles (AhRKO = 38,440 +/- 3632 versus wild-type = 21,120 +/- 2688) and fewer single germ cells than wild-type mice (AhRKO = 12,696 +/- 1192 vs. wild-type = 18,160 +/- 720). On PND 8 and 32-35, there was no difference in the number of follicles between AhRKO and wild-type mice but by PND 53, AhRKO mice had significantly fewer antral follicles than wild-type (AhRKO = 3416 +/- 480 vs, wild-type = 6776 +/- 1024). Taken together, these results suggest that the AhR may play a role in the formation of primordial follicles and the regulation of antral follicle numbers.