Multiple transcripts of MUC3:: Evidence for two genes, MUC3A and MUC3B
Multiple transcripts of MUC3:: Evidence for two genes, MUC3A and MUC3B
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DOI：
10.1006/bbrc.2000.3406
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发表时间：
2000-09-07
影响因子：
3.1
通讯作者：
Swallow, DM
中科院分区：
文献类型：
--
作者：
Pratt, WS;Crawley, S;Swallow, DM
MUC 3基因编码跨膜粘蛋白型糖蛋白，在来自单个个体的不同MUC 3 cDNA中观察到许多一致的单核苷酸变化，表明每个个体存在至少三种不同的转录本，这种转录本异质性是由于MUC 3基因的第二拷贝的存在和等位基因变化。第二个MUC 3的测序表明，它具有与先前描述的MUC 3相同的C-末端结构域和内含子/外显子结构，串联重复结构域具有相同的氨基酸共有序列，但显示更多的取代，独特的外显子序列在核苷酸水平上的同一性范围为94%至100%，相应地，已经鉴定出很少的氨基酸变化。内含子显示约95%的同一性。我们建议将这第二个基因命名为MUC 3B，MUC 3A保留用于第一个MUC 3基因。与MUC 3A一样，MUC 3B在肠和Caco-2细胞中表达。(C)北京大学出版社.
The MUC3 gene encodes a transmembrane mucin-type glycoprotein, A number of consistent single nucleotide changes were observed in different MUC3 cDNAs from a single individual, suggesting the presence of at least three different transcripts per individual, This transcript heterogeneity is due both to the existence of a second copy of the MUC3 gene and to allelic changes. Sequencing of the second MUC3 shows that it has the same C-terminal domain and intron/exon structure as the previously described MUC3, The tandem repeat domain has the same amino acid consensus sequence but shows more substitutions, The unique exonic sequences range from 94 to 100% identity at the nucleotide level and correspondingly few amino acid changes have been identified. The introns show around 95% identity. We propose to name this second gene MUC3B, MUC3A being reserved for the first MUC3 gene. MUC3B, like MUC3A, is expressed in intestine and Caco-2 cells. (C) 2000 Academic Press.