Two olfactory placode derived galanin subpopulations: luteinizing hormone-releasing hormone neurones and vomeronasal cells.
Two olfactory placode derived galanin subpopulations: luteinizing hormone-releasing hormone neurones and vomeronasal cells.
复制标题
两个嗅基板衍生的甘丙肽亚群：黄体生成素释放激素神经元和犁鼻细胞。
DOI：
10.1046/j.1365-2826.2000.00486.x
复制
发表时间：
2000
影响因子：
3.2
通讯作者：
Wray,S
中科院分区：
文献类型：
--
作者：
Key,S;Wray,S
在成年啮齿动物中，肽甘丙肽以活动依赖的方式在下丘脑黄体生成素释放激素（LHRH）神经元亚群中表达。在这项研究中，我们研究了小鼠甘丙氨酸mRNA的表达是否与LHRH mRNA的表达一致，因为LHRH神经元与嗅觉基因区不同。使用原位杂交，我们发现(i)甘丙氨酸mRNA在产前在LHRH神经元中共表达，（ii）当LHRH mRNA阳性/甘丙氨酸mRNA阳性的神经元从嗅窝迁移到鼻中隔时，甘丙氨酸mRNA的表达相对于LHRH mRNA的表达有所减少。（iii）在嗅窝/犁鼻器官中存在一种新的甘丙氨酸mRNA阳性/LHRH mRNA阴性表达的神经元群体，这些神经元不会迁移到中枢神经系统（CNS）。本研究表明，至少有两个群体的甘丙氨酸mRNA表达神经元产生于嗅觉基因座；一种保留在鼻腔区域，LHRH mRNA阴性且功能未知，另一种与LHRH共表达。此外，甘丙肽mRNA在LHRH细胞中的时间表达表明，甘丙肽mRNA表达的初始激活和随后的失活与中枢神经系统突触连接无关。
In adult rodents, the peptide galanin is expressed in a subpopulation of hypothalamic luteinizing hormone‐releasing hormone (LHRH) neurones in an activity‐dependent manner. In this investigation, we examined whether galanin mRNA expression in mice was activated coincident with LHRH mRNA expression, as LHRH neurones differentiate from the olfactory placode. Usingin situhybridization, we show (i) that galanin mRNA is coexpressed in LHRH neurones prenatally, (ii) that there is a decrease in galanin mRNA expression relative to LHRH mRNA expression once LHRH mRNA positive/galanin mRNA positive neurones migrate out of the olfactory pit and into the nasal septum, and (iii) the presence of a novel population of galanin mRNA positive/LHRH mRNA negative expressing neurones in the olfactory pit/vomeronasal organ which do not migrate into the central nervous systenm (CNS). This study demonstrates that there are at least two populations of galanin mRNA expressing neurones arising from the olfactory placode; one that remains in nasal regions, is LHRH mRNA negative and whose function is unknown, and one which is coexpressed with LHRH. In addition, the temporal expression of galanin mRNA in LHRH cells indicates that initial activation and subsequent inactivation of galanin mRNA expression is independent of synaptic CNS connections.