Impact of anesthesia, sex, and circadian cycle on renal afferent nerve sensitivity.
Impact of anesthesia, sex, and circadian cycle on renal afferent nerve sensitivity.
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麻醉、性别和昼夜节律对肾传入神经敏感性的影响。
DOI：
10.1152/ajpheart.00675.2020
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Stocker,SeanD
中科院分区：
文献类型：
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作者：
DeLalio,LeonJ;Stocker,SeanD
肾传入神经(ARNA)活性升高或肾-肾反射功能障碍通过ARNA敏感性改变导致高血压和慢性肾脏疾病。这些神经含有对缺血、肾内压力变化和趋化因子作出反应的机械和化学敏感纤维。大多数研究都利用各种麻醉制剂和专门的雄性动物来表征Arna的反应。因此，本研究评估了麻醉、性别和昼夜节律对ARNA反应和敏感性的影响。在雄性和雌性SD大鼠(250-400 g)上进行多纤维ARNA记录，并在去大脑与肌动蛋白、异氟醚和乌拉坦麻醉组之间进行比较。肾动脉内注入辣椒素(0.1~5 0.0 μM，0.0 5 m L)可引起ARNA的浓度依赖性增加；然而，去大脑的ARNA敏感性显著高于肌动素组、异氟醚和氨基甲酸乙酯组。肾盆压力的增加(0-30 ，S 30 mmHg)导致ARNA的压力依赖性增加；然而，去大脑和Inactin组的ARNA敏感性再次高于异氟醚和氨基甲酸乙酯。急性肾动脉阻断(30 S)使ARNA升高，但不同组间的反应没有差异。对雄性和雌性大鼠对盆腔压力升高的ARNA反应的分析表明，只有异氟醚组和氨基甲酸酯组存在显著的性别差异。ARNA对肾内注射辣椒素的反应在夜间与白天相比明显减弱；然而，ARNA对盆腔压力增加或肾动脉闭塞的反应在白天和夜间没有区别。这些结果表明，去大脑和非肌动麻醉组的ARNA敏感性最高，但并不总是受到性别的影响。我们确定了麻醉、性别和昼夜周期对肾传入神经(ARNA)对化学和机械刺激的敏感性的影响。ARNA对肾脏辣椒素输注的敏感性在去大脑、Inactin和GT、氨基甲酸酯或异氟醚组最高。肾盆压力升高显著增加ARNA；去脑组和Inactin组对ARNA的敏感性最高。肾传入反应的性别差异并不一致。昼夜节律改变了化学感觉，但不改变机械感觉反应。
Elevated renal afferent nerve (ARNA) activity or dysfunctional reno-renal reflexes via altered ARNA sensitivity contribute to hypertension and chronic kidney disease. These nerves contain mechano- and chemosensitive fibers that respond to ischemia, changes in intrarenal pressures, and chemokines. Most studies have utilized various anesthetized preparations and exclusively male animals to characterize ARNA responses. Therefore, this study assessed the impact of anesthesia, sex, and circadian period on ARNA responses and sensitivity. Multifiber ARNA recordings were performed in male and female Sprague-Dawley rats (250–400 g) and compared across decerebrate versus Inactin, isoflurane, and urethane anesthesia groups. Intrarenal artery infusion of capsaicin (0.1–50.0 μM, 0.05 mL) produced concentration-dependent increases in ARNA; however, the ARNA sensitivity was significantly greater in decerebrate versus Inactin, isoflurane, and urethane groups. Increases in renal pelvic pressure (0–30 mmHg, 30 s) produced pressure-dependent increases in ARNA; however, ARNA sensitivity was again greater in decerebrate and Inactin groups versus isoflurane and urethane. Acute renal artery occlusion (30 s) increased ARNA, but responses did not differ across groups. Analysis of ARNA responses to increased pelvic pressure between male and female rats revealed significant sex differences only in isoflurane and urethane groups. ARNA responses to intrarenal capsaicin infusion were significantly blunted at nighttime versus daytime; however, ARNA responses to increased pelvic pressure or renal artery occlusion were not different between daytime and nighttime. These results demonstrate that ARNA sensitivity is greatest in decerebrate and Inactin-anesthetized groups but was not consistently influenced by sex.NEW & NOTEWORTHYWe determined the impact of anesthesia, sex, and circadian cycle on renal afferent nerve (ARNA) sensitivity to chemical and mechanical stimuli. ARNA sensitivity to renal capsaicin infusion was greatest in decerebrate > Inactin > urethane or isoflurane groups. Elevated renal pelvic pressure significantly increased ARNA; decerebrate and Inactin groups exhibited the greatest ARNA sensitivity. Sex differences in renal afferent responses were not consistently observed. Circadian cycle altered chemosensory but not mechanosensory responses.