MultiLocus Sequence Analysis- and Amplified Fragment Length Polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species
MultiLocus Sequence Analysis- and Amplified Fragment Length Polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species
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DOI：
10.1016/j.syapm.2011.12.005
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Pruvost, O.
中科院分区：
文献类型：
--
作者：
Hamza, A. A.;Robene-Soustrade, I.;Pruvost, O.
采用多位点序列分析（MLSA）和扩增片段长度多态性（AFLP）方法，对综合收集的单胞菌病原菌与单胞菌的遗传亲缘关系进行了分析。MLSA方案基于四个管家基因（atpD, dnaK， efp和gyrB）的部分序列。在全球范围内，MLSA数据在物种水平上明确地鉴定出番茄和辣椒的细菌性斑疹菌株，与AFLP数据一致。两种技术的遗传距离表明：(1)紫花蓟马、穿孔蓟马和紫花蓟马的亲缘关系较近；(2)加特纳蓟马和cynarae的亲缘关系较近。从每个基因部分得到的最大似然树拓扑与X. campestris 16S rRNA核心的物种（即包括引起番茄和辣椒细菌性斑疹的所有菌株的物种簇）的串联数据集不一致，这与我们通过几种重组搜索算法在数据集中检测到的几个假定的重组事件一致。在包括型菌株在内的大多数猪瘟弧菌株中均鉴定出一个atpD重组区。(C) 2012 Elsevier GmbH版权所有。
MultiLocus Sequence Analysis (MLSA) and Amplified Fragment Length Polymorphism (AFLP) were used to measure the genetic relatedness of a comprehensive collection of xanthomonads pathogenic to solaneous hosts to Xanthomonas species. The MLSA scheme was based on partial sequences of four housekeeping genes (atpD, dnaK, efp and gyrB). Globally, MLSA data unambiguously identified strains causing bacterial spot of tomato and pepper at the species level and was consistent with AFLP data. Genetic distances derived from both techniques showed a close relatedness of (i) X. euvesicatoria, X. perforans and X. alfalfae and (ii) X. gardneri and X. cynarae. Maximum likelihood tree topologies derived from each gene portion and the concatenated data set for species in the X. campestris 16S rRNA core (i.e. the species cluster comprising all strains causing bacterial spot of tomato and pepper) were not congruent, consistent with the detection of several putative recombination events in our data sets by several recombination search algorithms. One recombinant region in atpD was identified in most strains of X. euvesicatoria including the type strain. (C) 2012 Elsevier GmbH. All rights reserved.